Description
Xanthine oxidase is a molybdenum-containing enzyme that is found in the cytosol, and may be strongly inhibited by flavonoids. It plays a vital role in the metabolism of some drugs, as well as purines and pyrimidines. It is also known to be a biological source of reactive oxygen species. Xanthine oxidase was shown to be involved in the reduction of cytochrome c by the generation of superoxide anions following the oxidation of xanthine. These free radicals are responsible for reducing cytochrome c.
Product Overview
Formerly E.C. 1.1.3.22
Form
Type I, Type II, ammonium sulfate suspension; Suspension in 2.3 M (NH4)2SO4 containing 1 mM sodium salicylate; Type III, ammonium sulfate suspension, Suspension in 2.3 M (NH4)2SO4, 10 mM sodium phosphate buffer, pH 7.8, containing 1 mM EDTA and 1 mM sodium salicylate; Type IV, lyophilized powder, Contains 0.5% sodium salicylate.
Enzyme Commission Number
EC 1.17.3.2.
Activity
Type I, > 0.4 units/mg protein; Type II, 0.1-0.4 units/mg protein; Type III, 1.0-2.0 units/mg protein; Type IV, 0.4-1.0 units/mg protein.
Unit Definition
One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25°C. Approx. 50% of the activity is obtained with hypoxanthine as substrate.
Warnings
Protein determined by biuret
Synonyms
Xanthine oxidase; XO; xanthine oxidoreductase; EC 1.17.3.2; 9002-17-9; XOD; Xanthine:oxygen oxidoreductase; hypoxanthine oxidase; hypoxanthine:oxygen oxidoreductase; Schardinger enzyme; hypoxanthine-xanthine oxidase; xanthine:O2 oxidoreductase; xanthine:xanthine oxidase
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