Description
Xanthine oxidase is a molybdenum-containing enzyme that is found in the cytosol, and may be strongly inhibited by flavonoids. It plays a vital role in the metabolism of some drugs, as well as purines and pyrimidines. It is also known to be a biological source of reactive oxygen species. Xanthine oxidase was shown to be involved in the reduction of cytochrome c by the generation of superoxide anions following the oxidation of xanthine. These free radicals are responsible for reducing cytochrome c.
Abbr
XO, Native (Microbial)
Applications
This enzyme is useful for enzymatic determination of inorganic phosphorus, 5′-nucleotidase and adenosine deaminase when coupled with Purine-nucleoside phosphorylase and uricase.
Product Overview
Allopurinol is a synthetic drug show to inhibit xanthine oxidase.
Form
Lyophilized powder containing BSA and sodium glutamate as stabilizers
Enzyme Commission Number
EC 1.17.3.2.
Activity
> 7 units/mg solid
Molecular Weight
mol wt ~160 kDa
pH Stability
pH 6.5-9.0 (25°C, 15hr)
Unit Definition
One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25°C. Approx. 50% of the activity is obtained with hypoxanthine as substrate.
Thermal stability
below 55°C (pH 8.0, 30min)
Inhibitors
Reducing agents, Hg++, Ag+,MIA
Synonyms
Xanthine oxidase; XO; xanthine oxidoreductase; EC 1.17.3.2; 9002-17-9; XOD; Xanthine:oxygen oxidoreductase; hypoxanthine oxidase; hypoxanthine:oxygen oxidoreductase; Schardinger enzyme; hypoxanthine-xanthine oxidase; xanthine:O2 oxidoreductase; xanthine:xanthine oxidase
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