Description
In enzymology, a N-acetylneuraminate lyase (EC 4.1.3.3) is an enzyme that catalyzes the chemical reaction: N-acetylneuraminate ↔ N-acetyl-D-mannosamine + pyruvate. Hence, this enzyme has one substrate, N-acetylneuraminate, and two products, N-acetyl-D-mannosamine and pyruvate. This enzyme belongs to the family of lyases, specifically the oxo-acid-lyases, which cleave carbon-carbon bonds.
Abbr
NALase (Microorganism)
Alias
NPL; NALase; NANA lyase
Applications
This enzyme is useful for enzymatic determination of N-acetylneuraminic acid and sialic acid when coupled with the related enzymes in clinical analysis. For industrial use, this enzyme is useful for enzymatic synthesis of sialic acid.
Appearance
Yellowish amorphous powder, lyophilized
Enzyme Commission Number
EC 4.1.3.3
Activity
Grade III 15U/mg-solid or more (30U/mg-protein or more), (containing approx. 30% of stabilizers)
Contaminants
Catalase < 1.0%, NADH oxidase < 1.0x10⁻³%
Molecular Weight
approx. 98 kDa
Isoelectric point
4.6±0.1
pH Stability
pH 6.0−9.0 (10°C, 25hr)
Michaelis Constant
2.5×10⁻³M (N-Acetylneuraminic acid)
Structure
3 subunits (approx. 35,000) per mol of enzyme
Thermal stability
below 65°C (pH 7.5, 30min)
Stability
Stable at-20°C for at least 6 months
Inhibitors
p-Chloromercuribenzoate, sodium dodecyl sulfate, Hg⁺⁺, Ag⁺
Synonyms
N-Acetylneuraminate Pyruvate Lyase; N-Acetylneuraminic Acid Lyase; NANA Aldolase; EC 4.1.3.3; N-acetylneuraminate pyruvate-lyase (N-acetyl-D-mannosamine-forming); N-acetylneuraminic acid aldolase; acetylneuraminate lyase; sialic aldolase; sialic acid aldolase; sialate lyase; N-acetylneuraminic aldolase; neuraminic aldolase; N-acetylneuraminate aldolase; neuraminic acid aldolase; N-acetylneuraminic acid aldolase; neuraminate aldolase; N-acetylneuraminic lyase; NPL; NALase; NANA lyase; acetylneuraminate pyruvate-lyase; N-acetylneuraminate pyruvate-lyase
Download Datasheet