Description
In enzymology, a N-acetylneuraminate lyase (EC 4.1.3.3) is an enzyme that catalyzes the chemical reaction:N-acetylneuraminate↔ N-acetyl-D-mannosamine + pyruvate. Hence, this enzyme has one substrate, N-acetylneuraminate, and two products, N-acetyl-D-mannosamine and pyruvate. This enzyme belongs to the family of lyases, specifically the oxo-acid-lyases, which cleave carbon-carbon bonds. This enzyme participates in aminosugars metabolism.
Abbr
NPL, Native (Escherichia coli)
Alias
NPL; NALase; NANA lyase
Applications
This enzyme is useful for enzymatic determination of N-acetylneuraminic acid and sialic acid when coupled with the related enzymes in clinical analysis. For industrial use, this enzyme is useful for enzymatic synthesis of sialic acid. Used in the Sialic Acid Quantification Kit, SIALIC-Q
Form
Lyophilized powder containing potassium phosphate buffer salt
Enzyme Commission Number
EC 4.1.3.3
Activity
> 20 units/mg protein (biuret)
Molecular Weight
mol wt ~98 kDa
Isoelectric point
4.6 ± 0.1
pH Stability
pH 6.0–9.0 (10°C, 25hr)
Michaelis Constant
2.5 x 10‾3M (N-Acetylneuraminic acid)
Structure
3 subunits (approx. 35 kDa) per mol of enzyme
Unit Definition
One unit will release 1.0 μmole of pyruvate from NANA per min at pH 7.7 at 37°C.
Thermal stability
Below 65°C (pH 7.5, 30 min)
Inhibitors
p-Chloromercuribenzoate, sodium dodecyl sulfact, Hg++, Ag+
Synonyms
N-acetylneuraminic acid aldolase; acetylneuraminate lyase; sialic aldolase; sialic acid aldolase; sialate lyase; N-acetylneuraminic aldolase; neuraminic aldolase; N-acetylneuraminate aldolase; neuraminic acid aldolase; N-acetylneuraminic acid aldolase; neuraminate aldolase; N-acetylneuraminic lyase; N-acetylneuraminic acid lyase; NPL; NALase; NANA lyase; acetylneuraminate pyruvate-lyase; N-acetylneuraminate pyruvate-lyase; 9027-60-5; EC 4.1.3.3
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