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Native Glycine max (soybean) Lipoxidase

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Activity

Size

Price

Qty

Activity10 U/mg protein

Size4 g

Price$298

Qty

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Cat No.

NATE-0407

Description

Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure.

Abbr

Lipoxygenase, Native (Glycine max (soybean))

Source

Glycine max (soybean)

Applications

The soybean enzyme will use arachidonic acid as a substrate, with ~ 15% of the activity indicated using linoleic acid as the substrate; the product of arachidonic acid oxidation is 12-or 15-hydroperoxyarachidonic acid (12-HPETE or 15-HPETE).

Form

Type I, ammonium sulfate suspension, Suspension in 2.3 M (NH4)2SO4 solution, pH approx. 6.0; Type II, lyophilized powder, Contains stabilizer and NaCl.

Enzyme Commission Number

EC 1.13.11.12

Activity

Type I, 500,000-1,000,000 units/mg protein; Type II, > 50,000 units/mg solid.

CAS No.

9029-60-1

Molecular Weight

mol wt 108 kDa (two 54 kDa subunits)

Unit Definition

One unit will cause an increase in A234 of 0.001 per min at pH 9.0 at 25°C when linoleic acid is the substrate in 3.0 ml volume (1 cm light path). One A234 unit is equivalent to the oxidation of 0.12 μmole of linoleic acid.

Storage

2-8°C

Warnings

Prepared by affinity chromatography. Protein determined by biuret.

Synonyms

Lipoxygenases; EC 1.13.11.12; 9029-60-1; 13-lipoxidase; carotene oxidase; 13-lipoperoxidase; fat oxidase; 13-lipoxydase; lionoleate:O2 13-oxidoreductase; linoleate 13S-lipoxygenase

"Lipoxygenase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-1248	15-Lipoxygenase-2 from human, Recombinant	EC 1.13.11.33		E. coli	Inquiry
NATE-1249	5-Lipoxygenase from human, Recombinant	EC 1.13.11.34	80619-02-9	S9 insect cells	Inquiry
EXWM-0532	linoleate 13S-lipoxygenase	EC 1.13.11.12	9029-60-1		Inquiry
EXWM-0552	arachidonate 15-lipoxygenase	EC 1.13.11.33	82249-77-2		Inquiry

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