Description
Superoxide dismutase (SOD) catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. SOD plays a critical role in the defense of cells against the toxic effects of oxygen radicals. SOD competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice by preventing the conversion of NO to peroxynitrate, an inducer of apoptosis.
Abbr
SOD, Native (E. coli)
Applications
The Cu,Zn superoxide dismutase from Escherichia coli retains monomeric structure at high protein concentration, which could be applied towards future assessments of altered subunit interaction in all the bacteri ocupreins. Superoxide dismutase from Escherichia coli has also been used in a study to investigate the superoxide anion participattion in 2-oxoglutarate-dependent hydroxylation.
Product Overview
Superoxide dismutases are a group of low molecular weight metalloproteins present in all aerobic cells of plants, animals and micro-organisms. They provide protection against damaging reactions with the superoxide radical anion (O2-) by catalyzing its disproportionation into oxygen and hydrogen peroxide.
Form
Lyophilized powder containing Tris buffer salts
Enzyme Commission Number
EC 1.15.1.1
Activity
> 1 ,000 units/mg protein
Molecular Weight
mol wt 32.5 kDa
Unit Definition
One unit will inhibit reduction of Cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25°C in a 3.0 mL reaction volume. Xanthine oxidase concentration should produce an initial ΔA550 of 0.025 ± 0.005 per min.
Warnings
Iron-containing enzyme; For assay method, see McCord, J.M. and Fridovich,I., J. Biol. Chem., 244, 6049 (1969).
Synonyms
Superoxide dismutases; EC 1.15.1.1; superoxidase dismutase; copper-zinc superoxide dismutase; Cu-Zn superoxide dismutase; ferrisuperoxide dismutase; superoxide dismutase I; superoxide dismutase II; SOD; Cu,Zn-SOD; Mn-SOD; Fe-SOD; SODF; SODS; SOD-1; SOD-2; SOD-3; SOD-4; hemocuprein; erythrocuprein; cytocuprein; cuprein ; hepatocuprein; 9054-89-1
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