Description
Superoxide dismutase (SOD) catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. SOD plays a critical role in the defense of cells against the toxic effects of oxygen radicals. SOD competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice by preventing the conversion of NO to peroxynitrate, an inducer of apoptosis.
Abbr
SOD, Native (Bacillus stearothermophilus)
Source
Bacillus stearothermophilus
Applications
The enzyme is useful for medicine, cosmetic material and nutrition or antioxidant.
Enzyme Commission Number
EC 1.15.1.1
Contaminants
(as SOD activity = 100 %)
Catalase: < 0.01 %
Molecular Weight
ca. 50,000; Subunit molecular weight : ca. 25,000.
Unit Definition
One unit of activity is defined as the amount of SOD required to inhibit the rate of reduction of cytochrome C by 50 % at 30 °C.
Thermal stability
No detectable decrease in activity up to 60 °C.
Storage
Stable at -20 °C for at least one year.
Synonyms
Superoxide dismutases; EC 1.15.1.1; superoxidase dismutase; copper-zinc superoxide dismutase; Cu-Zn superoxide dismutase; ferrisuperoxide dismutase; superoxide dismutase I; superoxide dismutase II; SOD; Cu,Zn-SOD; Mn-SOD; Fe-SOD; SODF; SODS; SOD-1; SOD-2; SOD-3; SOD-4; hemocuprein; erythrocuprein; cytocuprein; cuprein ; hepatocuprein; 9054-89-1
Specific Activity
more than 9,000 U/mg protein
Reaction
O2- + O2- + 2H+ ←→ O2 + H2O2
Notes
Metal content: 1.5 g atoms of Mn per mole of enzyme.
Download Datasheet