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Native Escherichia coli L-Glutamine Synthetase
Cat No.
NATE-0321
Description
Glutamine synthetase (GS) (EC 6.3.1.2) is an enzyme that plays an essential role in the metabolism of nitrogen by catalyzing the condensation of glutamate and ammonia to form glutamine:Glutamate + ATP + NH3 → Glutamine + ADP + phosphate. Glutamine Synthetase uses ammonia produced by Nitrate reduction, amino acid degradation, and photorespiration. The amide group of glutamate is a nitrogen source for the synthesis of glutamine pathway metabolites.
Abbr
GS, Native (E. coli)
Alias
GS
Source
Escherichia coli
Applications
L-Glutamine synthetase may be used for the purification of proteases from Escherichia coli.
Form
lyophilized powder; Contains potassium phosphate, sodium Citrate and magnesium acetate buffer salts
Enzyme Commission Number
EC 6.3.1.2
Activity
400-2,000 units/mg protein
CAS No.
9023-70-5
Purity
affinity chromatography
Unit Definition
One unit will convert 1.0 μmole of L-glutamate to L-glutamine in 15 min at pH 7.1 at 37°C.
Storage
−20°C
Buffer
H2O: soluble 0.95-1.05 mg/mL, clear to hazy
Warnings
Grown in medium containing glucose and NH4Cl
Pathway
Alanine, aspartate and glutamate metabolism, organism-specific biosystem; Arginine and proline metabolism, organism-specific biosystem; Metabolic pathways, organism-specific biosystem
Synonyms
glutamine synthetase; glutamylhydroxamic synthetase; L-glutamine synthetase; glutamate-ammonia ligase; L-Glutamate:ammonia ligase (ADP-forming); EC 6.3.1.2; GS; 9023-70-5
Download
Download Datasheet| Catalog | Product Name | EC No. | CAS No. | Source | Price |
|---|---|---|---|---|---|
| NATE-1675 | Glutamine Synthetase from Human, recombinant | EC 6.3.1.2 | 9023-70-5 | E. coli | Inquiry |
| DIA-155 | Native Bacillus sp. Glutamine synthetase | EC 6.3.1.2 | 9023-70-5 | Bacillus sp. | Inquiry |
| DIA-411 | Native Glutamine Synthetase from Microorganism | EC 6.3.1.2 | 9023-70-5 | Microorganism | Inquiry |
| EXWM-5722 | glutamine synthetase | EC 6.3.1.2 | 9023-70-5 | Inquiry |