Description
Glutamine synthetase (GS) (EC 6.3.1.2) is an enzyme that plays an essential role in the metabolism of nitrogen by catalyzing the condensation of glutamate and ammonia to form glutamine:Glutamate + ATP + NH3 → Glutamine + ADP + phosphate. Glutamine Synthetase uses ammonia produced by Nitrate reduction, amino acid degradation, and photorespiration. The amide group of glutamate is a nitrogen source for the synthesis of glutamine pathway metabolites.
Abbr
GS, Native (E. coli)
Applications
L-Glutamine synthetase may be used for the purification of proteases from Escherichia coli.
Form
lyophilized powder; Contains potassium phosphate, sodium Citrate and magnesium acetate buffer salts
Enzyme Commission Number
EC 6.3.1.2
Activity
400-2,000 units/mg protein
Purity
affinity chromatography
Unit Definition
One unit will convert 1.0 μmole of L-glutamate to L-glutamine in 15 min at pH 7.1 at 37°C.
Buffer
H2O: soluble 0.95-1.05 mg/mL, clear to hazy
Warnings
Grown in medium containing glucose and NH4Cl
Pathway
Alanine, aspartate and glutamate metabolism, organism-specific biosystem; Arginine and proline metabolism, organism-specific biosystem; Metabolic pathways, organism-specific biosystem
Synonyms
glutamine synthetase; glutamylhydroxamic synthetase; L-glutamine synthetase; glutamate-ammonia ligase; L-Glutamate:ammonia ligase (ADP-forming); EC 6.3.1.2; GS; 9023-70-5
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