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Native Glutamine Synthetase from Microorganism
Cat No.
DIA-411
Description
Glutamine synthetase (GS) (EC 6.3.1.2) is an enzyme that plays an essential role in the metabolism of nitrogen by catalyzing the condensation of glutamate and ammonia to form glutamine: Glutamate + ATP + NH3 → Glutamine + ADP + phosphate. Glutamine Synthetase uses ammonia produced by nitrate reduction, amino acid degradation, and photorespiration. The amide group of glutamate is a nitrogen source for the synthesis of glutamine pathway metabolites.
Abbr
GS, Native (Microorganism)
Alias
GS
Source
Microorganism
Appearance
Light yellow lyophilizate
Enzyme Commission Number
EC 6.3.1.2
Activity
> 7 U/mg lyophilizate
CAS No.
9023-70-5
Contaminants
catalase < 0.5%
Molecular Weight
ca. 900 kDa
Isoelectric point
6.5
pH Stability
6.5–9.5
Michaelis Constant
1.5 x 10^-2 M (L-glutamate) 1.3 x 10^-4 M (ammonia) 8.7 x 10^-4 M (ATP)
Structure
57 kDa (SDS-PAGE)
Specificity
L-glutamate (100), D-glutamate (0.8), NH3 (100), NH2OH (12), ATP (100), GTP (2.5)
Unit Definition
One unit (U) is defined as the amount of enzyme which produces 1 μmol of phosphate per min at 37°C and pH 7.0.
Optimum pH
7
Optimum temperature
60°C
Activators
Mg2+, Mn2+
Thermal stability
below 40°C
Storage
at -20°C
Stabilizers
Sucrose
Inhibitors
methionine sulfoximine, carbamyl phosphate
Synonyms
Glutamine synthetase; GS; EC 6.3.1.2; Glutamate-ammonia ligase
Download
Download Datasheet| Catalog | Product Name | EC No. | CAS No. | Source | Price |
|---|---|---|---|---|---|
| NATE-1675 | Glutamine Synthetase from Human, recombinant | EC 6.3.1.2 | 9023-70-5 | E. coli | Inquiry |
| DIA-155 | Native Bacillus sp. Glutamine synthetase | EC 6.3.1.2 | 9023-70-5 | Bacillus sp. | Inquiry |
| NATE-0321 | Native Escherichia coli L-Glutamine Synthetase | EC 6.3.1.2 | 9023-70-5 | Escherichia coli | Inquiry |
| EXWM-5722 | glutamine synthetase | EC 6.3.1.2 | 9023-70-5 | Inquiry |