Description
Phospholipases A2 (PLA2s) EC 3.1.1.4 are enzymes that release fatty acids from the second carbon group of glycerol. This particular phospholipase specifically recognizes the sn-2 acyl bond of phospholipids and catalytically hydrolyzes the bond releasing arachidonic acid and lysophospholipids. Upon downstream modification by cyclooxygenases, arachidonic acid is modified into active compounds called eicosanoids. Eicosanoids include prostaglandins and leukotrienes, which are categorized as anti-inflammatory and inflammatory mediators.
Abbr
PLA2, Native (Crotalus)
Source
Crotalus adamanteus Venom
Product Overview
Phospholipase A2 is a member of the class of heat-stable, calcium-dependent enzymes catalyzing the hydrolysis of the 2-acyl bond of 3-n-phosphoglycerides. A chromatographically purified, dialyzed, lyophilized powder.
Enzyme Commission Number
EC 3.1.1.4
Activity
> 200 units per mg dry weight
Molecular Weight
30 kDa (Wells 1969).
Purity
chromatographically purified, dialyzed
Isoelectric point
4.55 and 4.40 for Aα and Aβ respectively (Saito 1962).
Specificity
Substrate specificity has been investigated (VanDeenen 1963).
Unit Definition
One Unit releases one micromole of acid from soybean lecithin per minute at 25°C, pH 8.9
Activators
Calcium ion (Dennis 1973).
Stability
The enzyme is stable at 90°C and pH 3.0 for at least five minutes. (Uthe 1971; Saito 1962).
Inhibitors
Zinc, barium, and manganese ions (Uthe 1971). Also see Golec et al. (1992).
Synonyms
EC 3.1.1.4; Phospholipases A2; PLA2s
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