Description
Alkaline phosphatase (ALP, ALKP, ALPase, Alk Phos) (EC 3.1.3.1) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. As the name suggests, alkaline phosphatases are most effective in an alkaline environment. It is sometimes used synonymously as basic phosphatase.
Abbr
ALP, Native (Chicken)
Source
Chicken Intestine.
Product Overview
It was from Chicken Intestine Partially purified. A dried powder. Used in the NF/USP dexamethasone phosphate assay.
Enzyme Commission Number
EC 3.1.3.1
Activity
> 0.9 units per mg dry weight (25°C pH 8.8
Purity
Partially purified
Composition
The enzyme is a zinc metallo-enzyme. Schüssler (1968) reports four isozymes. Chang and Moog (1972) found three isozymes in the enzyme from chicken duodenum.
Unit Definition
One Unit hydrolyzes 1µmole of o-carboxyphenol phosphate per minute at 25°C, pH 8.8.
Activators
Schüssler (1968) indicates activation by Mg2+. See Sivanaesan et al. (1991).
Stability
The lyophilized preparation is stable for 1-2 years at 2-8°C.
Inhibitors
Acidification to pH 4.5 reversibly inactivates the enzyme.
Pathway
Folate biosynthesis, organism-specific biosystem; Folate biosynthesis, conserved biosystem; Metabolic pathways, organism-specific biosystem
Synonyms
Alkaline phosphatase; ALP; ALKP; ALPase; Alk Phos; EC 3.1.3.1; Alkaline phosphomonoesterase; Glycerophosphatase; Phosphomonoesterase
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