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Alkaline phosphatase (placental) from Human , Recombinant
Cat No.
NATE-1664
Description
Alkaline phosphatase (ALP, ALKP, ALPase, Alk Phos) (EC 3.1.3.1) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. As the name suggests, alkaline phosphatases are most effective in an alkaline environment. It is sometimes used synonymously as basic phosphatase.
Abbr
ALP, Recombinant (Human)
Source
Baculovirus
Species
Human
Form
Liquid
Enzyme Commission Number
EC 3.1.3.1
Activity
> 2,500 units/mg
Molecular Weight
This protein is fused with 6x His tag at C terminus (23-506 aa) and the protein has a calculated MW of 53.9 kDa (118aa). The protein migrates at 50-70KDa in SDS-PAGE under reducing conditions.
Purity
> 95% by SDS-PAGE
Concentration
0.5 mg/ml
Unit Definition
One unit is defined as the amount of enzyme that hydrolyzes 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.
Storage
Store at +4°C for short term (1-2 weeks). For long term storage, aliquot and store at -70°C. Avoid repeated freeze/thaw cycles.
Synonyms
ALPP; Alkaline phosphatase Regan isozyme; Placental alkaline phosphatase 1; PLAP-1
Endotoxin Level
< 1.0 EU per 1μg of protein
Download
Download Datasheet| Catalog | Product Name | EC No. | CAS No. | Source | Price |
|---|---|---|---|---|---|
| NATE-1871 | Native Bovine Alkaline Phosphatase | EC 3.1.3.1 | 9001-78-9 | Bovine Liver | Inquiry |
| NATE-1634 | Alkaline phosphatase from Mouse, Recombinant | EC 3.1.3.1 | Insect cell (Baculovirus) and fused to His-tag at C-terminus | Inquiry | |
| DIA-181 | Native Microorganism Alkaline phosphatase | EC 3.1.3.1 | 9001-78-9 | Microorganism | Inquiry |
| NATE-0053 | Native Bovine Alkaline Phosphatase | EC 3.1.3.1 | 9001-78-9 | Bovine intestinal mucosa | Inquiry |