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Mutant Isocitrate Dehydrogenase 1 R132H from Human, Recombinant

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Cat No.

NATE-1649

Description

Mutations of the cytosolic IDH 1 are a common feature in primary human brain cancers. Arginine 132 (R132) of IDH is highly conserved among different isoforms of IDH and is most commonly mutated to Histidine. Mutation of IDH1 R132H leads to accumulation of R(-)-2-hydroxyglutarate (2HG), which correlates with an increased risk for malignant brain tumors.

Abbr

IDH 1 R132H, Recombinant (Human)

Source

E. coli

Species

Human

Product Overview

Full-length human IDH1 (452 amino acids) with N-terminal HATtag. Arginine 132 is mutated to histidine

Form

Lyophilized powder with additives

Activity

>500 mU/mg

Molecular Weight

50.9 kDa

Purity

> 90% by SDS-PAGE

Unit Definition

One unit is the amount of enzyme that will convert 1.0 µmole of NADPH to NADP+ per minute at pH 7.4 at 25°C.

Storage

Lyophilized protein is stable for 1 year at -20°C. Once reconstituted aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.

Synonyms

Isocitrate dehydrogenase [NADP] cytoplasmic mutant, R132H; IDH 1 R132H

Reconstitution

Resuspend lyophilized protein in 30% glycerol glycerol/water at 2 mg/ml and then further diluted in future applications.

Formulation

In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol.

"IDH" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-1103	Isocitrate dehydrogenase from Bacillus subtilis, Recombinant	EC 1.1.1.42	9028-48-2	Bacillus subtilis	Inquiry
NATE-0350	Native Porcine Isocitric Dehydrogenase (NADP)	EC 1.1.1.42	9028-48-2	Porcine heart	Inquiry
NATE-0351	Isocitrate Dehydrogenase (NADP+) from Yeast, Recombinant	EC 1.1.1.42	9028-48-2	Pichia pastoris	Inquiry
NATE-1029	Isocitrate Dehydrogenase (NADP+) from Bacteria, Recombinant	EC 1.1.1.42	9028-48-2	E. coli	Inquiry

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