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Endoglycosidase S from Streptococcus pyogenes, Recombinant

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Cat No.

NATE-1603

Description

Endoglycosidase S is specific for N-glycans attached on the Fc domain of IgGs. The N-glycan is cleaved after the first Glc-Nac. Reaction is mild and runs fast under physiological conditions without the need for co-factors or detergent. The enzyme only hydrolyze glycans from the Fc-domain of IgGs even if other glycosylated proteins are present in solution.

Abbr

Endo S, Recombinant (Streptocoocus pyogenes)

Source

E.coli

Species

Streptocoocus pyogenes

Appearance

White to light yellow powder

Form

Lyophilized powder with no preservatives added

Molecular Weight

110 kDa

Purity

> 95% homogeneity as determined by SDS-PAGE analysis.

Unit Definition

One unit digests > 95% of 1μg IgG when incubated in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4 at 37°C for 30min.

Optimum pH

6.5-7.5

Stability

The enzyme is reconstituted by addition of water and after reconstitution it is stable for 1 month at +4-8°C. The enzyme can be aliquoted and stored at -20°C for at least 6 months. The product is shipped at ambient temperature but should be stored at -20°C upon arrival.

Storage

at -20°C

Buffer

Cleavage buffer at pH 7.4 and at 37°C yields optimal enzyme activity. Many buffers at pH between 6-8 can be used but reaction conditions should be tested on a small amount before employing the enzyme at full scale.

Synonyms

Endoglycosidase S; IgGZERO

"Endo-β-N-acetylglucosaminidase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-1780	Endoglycosidase S (Low Endotoxin) from Streptococcus pyogenes, Recombinant			E. coli	Inquiry
NATE-0214	Endoglycosidase F1 from Elizabethkingia miricola, Recombinant	EC 3.2.1.96	231-791-2	E. coli	Inquiry
NATE-0215	Endoglycosidase F2 from Elizabethkingia miricola, Recombinant	EC 3.2.1.96	37278-88-9	E. coli	Inquiry
NATE-0216	Endoglycosidase F3 from Elizabethkingia miricola, Recombinant	EC 3.2.1.96	231-791-2	E. coli	Inquiry

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