Description
An Endoglycosidase is an enzyme that releases oligosaccharides from glycoproteins or glycolipids. It may also cleave polysaccharide chains between residues that are not the terminal residue, although releasing oligosaccharides from conjugated protein and lipid molecules is more common. It breaks the glycosidic bonds between two sugar monomer in the polymer. It is different from exoglycosidase that it does not do so at the terminal residue. Hence, it is used to release long carbohydrates from conjugated molecules. If an exoglycosidase were used, every monomer in the polymer would have to be removed, one by one from the chain, taking a long time. An endoglycosidase cleaves, giving a polymeric product.
Abbr
Endo F1, Recombinant (Elizabethkingia miricola)
Species
Elizabethkingia miricola
Applications
Cleaves asparagine-linked or free oligomannose and hybrid, but not complex, oligosaccharides. An Endoglycosidase is an enzyme that releases oligosaccharides from glycoproteins or glycolipids. It may also cleave polysaccharide chains between residues that are not the terminal residue, although releasing oligosaccharides from conjugated protein and lipid molecules is more common. It breaks the glycosidic bonds between two sugar monomer in the polymer. It is different from exoglycosidase that it does not do so at the terminal residue. Hence, it is used to release long carbohydrates from conjugated molecules. If an exoglycosidase were used, every monomer in the polymer would have to be removed, one by one from the chain, taking a long time. An endoglycosidase cleaves, giving a polymeric product.
Package
Supplied with 5× Reaction Buffer, 250 mM NaH2PO4, pH 5.5.
Form
buffered aqueous solution, Aseptically filled solution in 20 mM Tris-HCl pH 7.5
Enzyme Commission Number
EC 3.2.1.96
Activity
> 16 U/mg, buffered aqueous solution
Unit Definition
One unit will release N-linked oligosaccharides from 1 μmole of denatured Ribonuclease B in 1 minute at 37°C, pH 5.5.
Synonyms
Endoglycosidase; Endo F1; Endo-β-N-acetylglucosaminidase F1; Endoglycosidase F1 from Chryseobacterium meningosepticum; Endoglycosidase F1 from Elizabethkingia meningoseptica; Endoglycosidase F1 from Flavobacterium meningosepticum; Endoglycosidase F1; EC 3.2.1.96; 231-791-2
Download Datasheet