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Endoglycosidase S (Low Endotoxin) from Streptococcus pyogenes, Recombinant

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Cat No.

NATE-1780

Description

EndoS LE is an endoglycosidase for deglycosylation of IgG Fc-glycan moieties. IgGZERO® LE hydrolyzes Fcglycans on IgG of all human IgG subclasses and IgG from the following species: mouse, rat, monkey, sheep, goat, cow and horse. The enzyme has limited activity on high-mannose and hybrid- type glycans.
EndoS LE hydrolyzes the β1,4 linkage between the core GlcNAc residues in the Fc-glycan, leaving the innermost GlcNAc on the Fc. EndoS LE is a low endotoxin product,use endotoxin free material and solutions.
Physiological reaction conditions at pH 7. 4 and 37°C yields optimal enzyme activity. Other buffers and pH (6-8) are compatible with enzyme activity but the reaction conditions needs to be tested to ensure efficient deglycosylation. EndoS LE has a mass of 110 kDa and contains a His-tag. EndoS LE is for R&D use only.

Abbr

Endo S, Recombinant (Streptocoocus pyogenes)

Source

E. coli

Species

Streptocoocus pyogenes

Form

Lyophilized in 10 mM sodium phosphate, 150 mM NaCl, pH 7. 4, with no preservatives added.

Molecular Weight

110 kDa

Purity

> 95% homogeneity as determined by SDS-PAGE analysis.

Unit Definition

One unit deglycosylates > 95% of 1 μg human IgG when incubated in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4 at 37°C for 30 min.

Storage

It is shipped at ambient temperature. It should be stored at -20°C upon arrival. After reconstitution EndoS LE is stable for 1 month at +4-8°C.

Synonyms

Endoglycosidase S; IgGZERO

Endotoxin Level

< 0.2 EU per vial

"Endo-β-N-acetylglucosaminidase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-0214	Endoglycosidase F1 from Elizabethkingia miricola, Recombinant	EC 3.2.1.96	231-791-2	E. coli	Inquiry
NATE-0215	Endoglycosidase F2 from Elizabethkingia miricola, Recombinant	EC 3.2.1.96	37278-88-9	E. coli	Inquiry
NATE-0216	Endoglycosidase F3 from Elizabethkingia miricola, Recombinant	EC 3.2.1.96	231-791-2	E. coli	Inquiry
NATE-0217	Endoglycosidase H from Streptomyces plicatus, Recombinant	EC 3.2.1.96	37278-88-9	E. coli	Inquiry

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