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Endo-β-N-acetylglucosaminidase from Clostridium perfringens, Recombinant

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Cat No.

NATE-1203

Description

An Endoglycosidase is an enzyme that releases oligosaccharides from glycoproteins or glycolipids. It may also cleave polysaccharide chains between residues that are not the terminal residue, although releasing oligosaccharides from conjugated protein and lipid molecules is more common. It breaks the glycosidic bonds between two sugar monomer in the polymer. It is different from exoglycosidase that it does not do so at the terminal residue. Hence, it is used to release long carbohydrates from conjugated molecules. If an exoglycosidase were used, every monomer in the polymer would have to be removed, one by one from the chain, taking a long time. An endoglycosidase cleaves, giving a polymeric product.

Abbr

Endo-β-N-acetylglucosaminidase, Recombinant (Clostridium perfringens)

Source

Clostridium perfringens

Form

Supplied in 3.2 M ammonium sulphate

Enzyme Commission Number

EC 3.2.1.96

CAS No.

37278-88-9

Purity

> 95 % as judged by SDS-PAGE

Unit Definition

One unit is defined as the amount of enzyme required to hydrolyse >95 % of the glycoforms from 10 μg of RNaseB in a total reaction volume of 10 μL at 37°C in 60 min, where a non-denaturing reaction buffer comprises 50 mM sodium phosphate buffer, pH 6.0, containing 1 mg/mL RNaseB.

Optimum temperature

> 25°C

Storage

Store at 4°C (shipped at room temperature)

Synonyms

Endoglycosidase; Endo-β-N-acetylglucosaminidase; EC 3.2.1.96; 231-791-2

"Endo-β-N-acetylglucosaminidase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-1780	Endoglycosidase S (Low Endotoxin) from Streptococcus pyogenes, Recombinant			E. coli	Inquiry
NATE-0214	Endoglycosidase F1 from Elizabethkingia miricola, Recombinant	EC 3.2.1.96	231-791-2	E. coli	Inquiry
NATE-0215	Endoglycosidase F2 from Elizabethkingia miricola, Recombinant	EC 3.2.1.96	37278-88-9	E. coli	Inquiry
NATE-0216	Endoglycosidase F3 from Elizabethkingia miricola, Recombinant	EC 3.2.1.96	231-791-2	E. coli	Inquiry

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