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Aldolase A from Human, Recombinant

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Cat No.

NATE-1663

Description

Fructose bisphosphate aldolase A, also known as Aldolase A is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. It is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Deficiency has been associated with myopathy and hemolytic anemia. Recombinant human Aldolase A, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Abbr

Aldolase A, Recombinant (Human)

Source

E. coli

Species

Human

Form

Liquid

Enzyme Commission Number

EC 4.1.2.13

Activity

> 1.5 units/mg

Molecular Weight

41.5 kDa (384 aa, 1-364 aa + His Tag), confirmed by MALDI-TOF.

Purity

> 95% by SDS-PAGE

Unit Definition

One unit will convert 1.0 µmole of fructose 1, 6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3- phosphate per minute at pH 7.5 at 25°C.

Storage

Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Synonyms

Fructose bisphosphate Aldolase A; ALDOA; ALDA; GSD12

"Aldolase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-0048	Native Rabbit Aldolase	EC 4.1.2.13	9024-52-6	Rabbit muscle	Inquiry
NATE-0049	Native Spinach Aldolase	EC 4.1.2.13	9024-52-6	Spinach	Inquiry
NATE-1152	Native Fructose-bisphosphate aldolase from Thermophillic bacteria	EC 4.1.2.13	9024-52-6	Thermophillic bacteria	Inquiry
EXWM-4853	fructose-bisphosphate aldolase	EC 4.1.2.13	9024-52-6		Inquiry

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