Description
Fructose-bisphosphate aldolase (EC 4.1.2.13), often just aldolase, is an enzyme catalyzing a reversible reaction that splits the aldol, fructose 1,6-bisphosphate, into the triose phosphates dihydroxyacetone phosphate (DHAP) and glyceraldehyde 3-phosphate (G3P). Aldolase can also produce DHAP from other (3S,4R)-ketose 1-phosphates such as fructose 1-phosphate and sedoheptulose 1,7-bisphosphate. Gluconeogenesis and the Calvin cycle, which are anabolic pathways, use the reverse reaction. Glycolysis, a catabolic pathway, uses the forward reaction. Aldolase is divided into two classes by mechanism.
Abbr
Aldolase, Native (Thermophillic bacteria)
Source
Thermophillic bacteria
Applications
Carbon bond formation between dihydroxyacetone phosphate and linear aldehydes.
Enzyme Commission Number
EC 4.1.2.13
Unit Definition
One unit is defined as the amount of enzyme oxidizing 1 μmol of NADH (ε340=6.22 mM-1cm-1 ) per 1 minute using fructose 1,6-bisphosphate as a substrate.
Thermal stability
~100% stability for 1 hour at 100°C
Buffer
20 mM Tris-HCl (pH 7.5), 20 mM KCl
Synonyms
aldolase; fructose-1,6-bisphosphate triosephosphate-lyase; Fructose-bisphosphate aldolase; fructose diphosphate aldolase; D-fructose-1,6-bisphosphate D-glyceraldehyde-3-phosphate-lyase; EC 4.1.2.13; 9024-52-6
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