Description
Hydroxynitrile lyases (Oxynitrilases) catalyze the addition of HCN to aldehydes and ketones thereby forming α-hydroxynitriles, also known as cyanohydrins. Hydroxynitrile Lyase from Arabidopsis thaliana , is used to hydrolyze mandelonitrile.
Species
Arabidopsis thaliana
Applications
Hydroxynitrile lyases (Oxynitrilases) are used for various biotransformation processes and to convert a broad range of substrates. They may be used with bacterial nitrilases to generate biocatalysts.
Enzyme Commission Number
EC 4.1.2.11
Unit Definition
1 U corresponds to the amount of enzyme which converts 1 μmol mandelonitrile per minte at pH 5.0 and 25°C.
Synonyms
hydroxynitrile lyase; oxynitrilase; Sorghum hydroxynitrile lyase; (S)-4-hydroxymandelonitrile hydroxybenzaldehyde-lyase
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