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ChnAC

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Official Full Name
ChnAC
Background
Chondroitinase AC from Flavobacteriun heparinum is an eliminase that degrades chondroitin sulfates A and C, but not chondroitin sulfate B. The enzyme cleaves, via an elimination mechanism, both sulfated and non-sulfated polysaccharide chains that contain (1→4)-linkages between hexosamines and glucuronic acid residues. The reaction yields oligosaccharide products, mainly disaccharides, with unsaturated uronic acids that can be detected by UV spectroscopy at 232 nm.
Synonyms
chondroitinase (ambiguous); chondroitin sulfate lyase; chondroitin AC eliminase; chondroitinase AC; ChnAC; 9047-57-8; EC 4.2.2.5
Catalog Product Name EC No. CAS No. Source Price
NATE-1738 Chondroitinase AC from Flavobacterium heparinum, Recombinant EC 4.2.2.5 9047-57-8 E. coli Inquiry
EXWM-5104 chondroitin AC lyase EC 4.2.2.5 9047-57-8 Inquiry
NATE-0126 Native Flavobacterium heparinum Chondroitinase AC EC 4.2.2.5 9047-57-8 Flavobacterium ... Inquiry
Related Info

Related Reading

ChnAC, also known as Chitinase AC, is an enzyme that plays a crucial role in the degradation and modification of chitin, a polymer widely found in the cell walls of fungi, exoskeletons of arthropods, and shells of crustaceans. ChnAC is a key member of the chitinase family and displays remarkable versatility in its functions and applications.

Background

Chitin is the second most abundant biopolymer in nature after cellulose. Chitinases are responsible for hydrolyzing chitin into small molecules such as chitooligosaccharides and N-acetyl-D-glucosamine (GlcNAc).ChnAC belongs to the Glycoside Hydrolase 18 (GH18) family, which is of interest because of its diverse functions and potential applications.

Structure

ChnAC has a conserved catalytic domain unique to the GH18 family of chitinases. The catalytic domain consists of a substrate-binding cleft that consists of multiple modules, including an N-terminal CBM (chitin-binding module) and a catalytic module.The modular structure of ChnAC allows it to interact with a wide range of chitin substrates.

Functions

ChnAC performs different functions in various areas, including：

  • Chitin Degradation: ChnAC is primarily responsible for the enzymatic hydrolysis of chitin, breaking it down into smaller molecules. This process is essential for the recycling of chitin in the environment and for the acquisition of nutrients by chitin-degrading bacteria and fungi.
  • Pathogen Defense: ChnAC plays an important role in the defense mechanisms of plants and animals. It helps defend against fungal pathogens by degrading chitin in fungal cell walls, thereby preventing colonization and infection.
  • Biotechnology Applications: ChnAC's ability to degrade chitin has been used in various industries. It can be used in agriculture for the biological control of plant pathogens, for the production of chitosan, a valuable biopolymer, and for the production of potentially biologically active chito-oligosaccharides.

Challenge

  • Substrate Specificity: Given the complexity of the chitin structure, it is critical to understand and optimize the substrate specificity of ChnAC. Determining the precise binding modes and mechanisms of ChnAC to different chitin substrates will improve its efficiency and specificity.
  • Engineering Improved Variants: Modifying ChnAC through protein engineering approaches to improve specific key properties such as catalytic efficiency, substrate specificity and stability is a major challenge. Overcoming these hurdles will lead to wider application and industrial utilization of ChnAC.

Applications

  • Agriculture: ChnAC can be used in biocontrol strategies and is applied to crops to protect against pathogenic fungi. It degrades the chitin in the fungal cell wall, destroying its structural integrity and preventing disease.
  • Biomedical and Pharmaceutical Industries: ChnAC-derived chitosan shows potential in various biomedical applications such as wound healing, drug delivery systems and immunomodulation. The unique properties of chitooligosaccharides make them attractive for pharmaceutical research and development.

Future Outlook

ChnAC has great potential for future development:

  • Bioremediation: The application of ChnAC's chitin-degrading properties to bioremediation could help mitigate the environmental pollution caused by chitin-based materials, including waste from the aquaculture and seafood industries.
  • Industrial Enzymology: With the growing demand for sustainable and eco-friendly processes, ChnAC can be used in a variety of industrial applications, including textile manufacturing, wastewater treatment, and biofuel production.

Conclusion

ChnAC, as a multifunctional chitinase, has multiple functions and applications in agriculture, biomedicine, and industry. Understanding its structure, function, challenges, and future prospects will help researchers develop strategies to optimize its efficiency and expand its applications. Operational and engineering studies of ChnAC have the potential to revolutionize the chitin degradation process, thereby providing innovative solutions for waste management, bioremediation, and sustainable resource use.