Description
Chondroitinase AC from Flavobacterium heparinum is an enzyme that cleaves sulfated and non-sulfated polysaccharide chains with (1-4) linkages between hexosamines and glucuronic acid residues, by an elimination mechanism. The resulting oligosaccharide products are mainly disaccharides with unsatuRated uronic acids. Chondroitinase AC specifically degrades chondroitin sulfates A and C, but not chondroitin sulfate B (dermatan sulfate).
Abbr
ChnAC, Native (Flavobacterium heparinum)
Source
Flavobacterium heparinum
Applications
Chondroitinase AC from Creative Enzymes has been used for the large scale preparation of glycosaminoglycan (GAG) fractions during the study of structural and sequence motifs in dermatan sulfate.
Enzyme Commission Number
EC 4.2.2.5
Activity
0.5-1.5 units/mg solid (using chondroitin sulfate A as substrate, also cleaves chondroitin sulfate C)
Unit Definition
One unit will cause a ΔA232 of 1.0 per minute due to the release of unsaturated disaccharide from chondroitin sulfate A at pH 7.3 at 37°C. Reaction volume: 3.1 ml (light path 1 cm).
Buffer
0.02 M phosphate buffer: soluble (pH 7.0)
Synonyms
EC 4.2.2.5; 9047-57-8; chondroitin AC lyase; chondroitinase; chondroitin sulfate lyase; chondroitin AC eliminase; chondroitin AC lyase; chondroitinase AC; ChnAC
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