Description
Native amino peptidase M from porcine kidney. Metalloprotease that hydrolyzes N-terminal amino acids from almost all unsubstituted oligopeptides. Does not cleave X-Pro bonds or N-blocked amino acids. Useful in peptide sequencing. Inhibited by EDTA, bestatin, and amastatin.
Appearance
White crystalline suspension
Enzyme Commission Number
EC 3.4.11.2
Contaminants
Oxidases: < 3%
Unit Definition
One unit is defined as the amount of enzyme that will hydrolyze 1 µmol of Leu-pNA per min at 37°C, pH 7.2.
Stability
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 4 months at -20°C.
Synonyms
Aminopeptidase M
Shipping Conditions
Ambient Temperature Only
Formulation
Crystalline suspension in 3.2 M (NH₄)₂SO₄, 10 mM MgCl₂, 10 mM Tris-HCl, pH 7.5.
Specific Activity
> 10 units/mg protein
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