Description
Protein Kinase C (PKC) is a serine/threonine kinase that is activated intracellularly by signal transduction pathways that produce DAG from phosphatidylinositol diphosphate (PIP2) and phosphatidylcholine (PC) through the action of various activated phospholipases. Phorbol esters also stimulate PKC. At least 11 PKC isozymes have been identified that differ in primary structure, tissue distribution, subcellular localization, response to extracellular signals, and substrate specificity. The isozymes can be grouped into three subfamilies. Members of the first family require Ca2+ and phospholipid and include PKCα, βI, βII, and γ. Members of the second family are phospholipid-dependent but Ca2+-independent, and include PKCδ, ε, η, and θ. Members of the third family are not activated by either DAG or phorbol esters and include PKCξ, μ, and ι.
Abbr
PKCE, Recombinant (Human)
Source
baculovirus infected insect cells
Product Overview
>95% (SDS-PAGE), recombinant, expressed in baculovirus infected insect cells, buffered aqueous glycerol solution
Form
buffered aqueous glycerol solution
Enzyme Commission Number
EC 2.7.1.37
Molecular Weight
apparent mol wt 89-96 kDa
Unit Definition
One unit will transfer 1 nmol of phosphate to PKC ε substrate peptide in 1 min at pH 7.4 at 30°C.
Buffer
Solution in 20mM HEPES, pH 7.4; 2mM EDTA, 2 mM EGTA, 5mM DTT, 100 mM NaCl, 0.05% Triton X-100, and 50% glycerol
Pathway
B Cell Receptor Signaling Pathway, organism-specific biosystem; CDC42 signaling events, organism-specific biosystem; Calcium Regulation in the Cardiac Cell, organism-specific biosystem; DAG and IP3 signaling, organism-specific biosystem; Disease, organism-specific biosystem; Downstream signal transduction, organism-specific biosystem; Downstream signaling in naive CD8+ T cells, organism-specific biosystem
Function
ATP binding; SH3 domain binding; actin monomer binding; calcium-independent protein kinase C activity; enzyme activator activity; enzyme binding; ethanol binding; metal ion binding; nucleotide binding; protein kinase C activity; protein kinase binding; receptor activator activity; signal transducer activity
Synonyms
PRKCE; protein kinase C, epsilon; protein kinase C epsilon type; PKCE; nPKC-epsilon; Ca2+-activated phospholipid-dependent serine-threonine kinase, ε isozyme human; PKCε human; PKCε; EC 2.7.1.37
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