Description
Protein Kinase C (PKC) is a serine/threonine kinase that is activated intracellularly by signal transduction pathways that produce DAG from phosphatidylinositol diphosphate (PIP2) and phosphatidylcholine (PC) through the action of various activated phospholipases. Phorbol esters also stimulate PKC. At least 11 PKC isozymes have been identified that differ in primary structure, tissue distribution, subcellular localization, response to extracellular signals, and substrate specificity. The isozymes can be grouped into three subfamilies. Members of the first family require Ca2+ and phospholipid and include PKCα, βI, βII, and γ. Members of the second family are phospholipid-dependent but Ca2+-independent, and include PKCδ, ε, η, and θ. Members of the third family are not activated by either DAG or phorbol esters and include PKCξ, μ, and ι.
Abbr
PKCH, Recombinant (Human)
Source
baculovirus infected insect cells
Product Overview
> 90% (SDS-PAGE), recombinant, expressed in baculovirus infected insect cells, buffered aqueous glycerol solution
Form
buffered aqueous glycerol solution
Enzyme Commission Number
EC 2.7.1.37
Molecular Weight
mol wt 82-84 kDa by SDS-PAGE
Unit Definition
One unit will transfer 1 nmol of phosphate to PKC ε substrate peptide in 1 min at pH 7.4 at 30°C.
Buffer
Solution in 20 mM HEPES, pH 7.4, 2 mM EDTA, 2 mM EGTA, 5 mM DTT, 250 mM NaCl, 0.05% Triton X-100, and 50% glycerol.
Pathway
Calcium Regulation in the Cardiac Cell, organism-specific biosystem; Effects of PIP2 hydrolysis, organism-specific biosystem; Endothelins, organism-specific biosystem; G Protein Signaling Pathways, organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem; G alpha (z) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem
Function
ATP binding; enzyme binding; metal ion binding; nucleotide binding; protein kinase C activity
Synonyms
PRKCH; Ca2+-activated phospholipid-dependent serine-threonine kinase η isozyme human; PKCη human; PKCH; EC 2.7.1.37
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