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Polynucleotide Phosphorylase from Escherichia coli, Recombinant

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Cat No.

NATE-0608

Description

Polynucleotide phosphorylase (PNPase) is a bifunctional enzyme with a phosphorolytic 3' to 5' exoribonuclease activity and a 3'-terminal oligonucleotide polymerase activity. It is also involved in mRNA processing and degradation in bacteria, plants, and humans.

Abbr

PNPase, Recombinant (E. coli)

Alias

PNPase

Source

E. coli

Species

Escherichia coli

Applications

Polynucleotide phosphorylase (PNP) has been used in a study to show that spontaneous mutations resulting from replication errors are reduced in a PNP-deficient strain. It has also been used in a study to show that the absence of PNPase makes E. coli cells sensitive to UV, which suggests PNP has a role in survival of UV damage.

Form

Supplied as a solution in 20 mM Hepes buffer pH 7.9, 0.1 mM EDTA, 2 mM DTT, 12.5 mM MgCl2, 200 mM KCl, 21.4% (w/v) Glycerol

Enzyme Commission Number

EC 2.7.7.8

CAS No.

9014-12-4

Unit Definition

One unit will polymerize 1.0 μmole of ADP releasing 1.0 μmole of inorganic phosphate in 15 minutes, at pH 9.1 at 37°C.

Storage

−70°C

Synonyms

PNPase; nucleoside diphosphate:polynucleotidyl transferase; polyribonucleotide nucleotidyltransferase; polynucleotide phosphorylase; polyribonucleotide phosphorylase; EC 2.7.7.8; 9014-12-4

"Polynucleotide phosphorylase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-1908	Native Bacillus stearothermophilus Polynucleotide Phosphorylase	EC 2.7.7.8	9014-12-4	Bacillus stearothermophilus	Inquiry
NATE-0609	Polynucleotide phosphorylase from Human, Recombinant	EC 2.7.7.8	9014-12-4	E. coli	Inquiry
NATE-0610	Polynucleotide phosphorylase from Synechocystis sp., Recombinant	EC 2.7.7.8	9014-12-4	E. coli	Inquiry
EXWM-3292	polyribonucleotide nucleotidyltransferase	EC 2.7.7.8	9014-12-4		Inquiry

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