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Polynucleotide Phosphorylase from Escherichia coli, Recombinant
Cat No.
NATE-0608
Description
Polynucleotide phosphorylase (PNPase) is a bifunctional enzyme with a phosphorolytic 3' to 5' exoribonuclease activity and a 3'-terminal oligonucleotide polymerase activity. It is also involved in mRNA processing and degradation in bacteria, plants, and humans.
Abbr
PNPase, Recombinant (E. coli)
Alias
PNPase
Source
E. coli
Species
Escherichia coli
Applications
Polynucleotide phosphorylase (PNP) has been used in a study to show that spontaneous mutations resulting from replication errors are reduced in a PNP-deficient strain. It has also been used in a study to show that the absence of PNPase makes E. coli cells sensitive to UV, which suggests PNP has a role in survival of UV damage.
Form
Supplied as a solution in 20 mM Hepes buffer pH 7.9, 0.1 mM EDTA, 2 mM DTT, 12.5 mM MgCl2, 200 mM KCl, 21.4% (w/v) Glycerol
Enzyme Commission Number
EC 2.7.7.8
CAS No.
9014-12-4
Unit Definition
One unit will polymerize 1.0 μmole of ADP releasing 1.0 μmole of inorganic phosphate in 15 minutes, at pH 9.1 at 37°C.
Storage
−70°C
Synonyms
PNPase; nucleoside diphosphate:polynucleotidyl transferase; polyribonucleotide nucleotidyltransferase; polynucleotide phosphorylase; polyribonucleotide phosphorylase; EC 2.7.7.8; 9014-12-4
Download
Download Datasheet"Polynucleotide phosphorylase" Total Products Page
| Catalog | Product Name | EC No. | CAS No. | Source | Price |
|---|---|---|---|---|---|
| NATE-1908 | Native Bacillus stearothermophilus Polynucleotide Phosphorylase | EC 2.7.7.8 | 9014-12-4 | Bacillus stearothermophilus | Inquiry |
| NATE-0609 | Polynucleotide phosphorylase from Human, Recombinant | EC 2.7.7.8 | 9014-12-4 | E. coli | Inquiry |
| NATE-0610 | Polynucleotide phosphorylase from Synechocystis sp., Recombinant | EC 2.7.7.8 | 9014-12-4 | E. coli | Inquiry |
| EXWM-3292 | polyribonucleotide nucleotidyltransferase | EC 2.7.7.8 | 9014-12-4 | Inquiry |