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Native Bacillus stearothermophilus Polynucleotide Phosphorylase

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Cat No.

NATE-1908

Description

Polynucleotide phosphorylase (PNPase) is a bifunctional enzyme with a phosphorolytic 3' to 5' exoribonuclease activity and a 3'-terminal oligonucleotide polymerase activity. It is also involved in mRNA processing and degradation in bacteria, plants, and humans.

Abbr

PNPase, Native (Bacillus stearothermophilus)

Alias

PNPase

Source

Bacillus stearothermophilus

Applications

The enzyme is useful for the preparation of polyribonucleotide.

Appearance

Lyophilized

Enzyme Commission Number

EC 2.7.7.8

CAS No.

9014-12-4

Molecular Weight

300,000 - 340,000; Subunit molecular weight : ca. 85,000.

Isoelectric point

pH Stability

9.0 - 11.0

Michaelis Constant

(38 mM Tris-HCI buffer, pH 9.5, at 60 °C)
Poly A: 0.27 mM**;
KH2PO4: 3.0 mM;
**concentration of poly A was calculated as AMP concentration.

Unit Definition

One unit of activity is defined as the amount of PNPase that forms 1 μmol of ADP per hour at 60 °C by depolymerizing of Poly A.

Optimum pH

9.0 - 9.5

Thermal stability

No detectable decrease in activity up to 55 °C.

Storage

Stable at -20 °C for at least one year.

Synonyms

PNPase; nucleoside diphosphate:polynucleotidyl transferase; polyribonucleotide nucleotidyltransferase; polynucleotide phosphorylase; polyribonucleotide phosphorylase; EC 2.7.7.8; 9014-12-4

Specific Activity

more than 2,000 U/mg protein

Reaction

RNAn+1 + Pi ←→ RNAn + Nucleoside diphosphate

Notes

Effectors: cations and anions.

"Polynucleotide phosphorylase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-0608	Polynucleotide Phosphorylase from Escherichia coli, Recombinant	EC 2.7.7.8	9014-12-4	E. coli	Inquiry
NATE-0609	Polynucleotide phosphorylase from Human, Recombinant	EC 2.7.7.8	9014-12-4	E. coli	Inquiry
NATE-0610	Polynucleotide phosphorylase from Synechocystis sp., Recombinant	EC 2.7.7.8	9014-12-4	E. coli	Inquiry
EXWM-3292	polyribonucleotide nucleotidyltransferase	EC 2.7.7.8	9014-12-4		Inquiry

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