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PNGase A from Oryza sativa (rice), Recombinant
Cat No.
NATE-1941
Description
PNGase A cleaves N-linked glycans from high mannose, hybrid, and short complex oligosaccharides such as those found in plant and insect cells. PNGase A differs from PNGase F in that it cleaves N-linked glycans with or without α(1,3)-linked core fucose residues.
PNGase A is a recombinant amidase, which cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and short complex oligosaccharides such as those found in plant and insect cells from N-linked glycoproteins and glycopeptides. PNGase A differs from PNGase F in that it cleaves N-linked glycans with or without α(1,3)-linked core fucose residues.
PNGase A is a recombinant amidase, which cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and short complex oligosaccharides such as those found in plant and insect cells from N-linked glycoproteins and glycopeptides. PNGase A differs from PNGase F in that it cleaves N-linked glycans with or without α(1,3)-linked core fucose residues.
Source
Pichia pastoris
Species
Oryza sativa (rice)
Form
Storage Conditions: 50 mM NaCl, 20 mM Tris-HCl, 5 mM EDTA, (pH 7.5 @ 25°C)
Enzyme Commission Number
EC 3.5.1.52
Activity
5,000 units/ml
Molecular Weight
63.8 kDa
Purity
> 95% pure as determined by SDS-PAGE
Unit Definition
One unit is defined as the amount of enzyme required to remove > 95% of the carbohydrate from 1 µg of denatured recombinant Avidin produced in Maize in 1 hour at 37°C in a total reaction volume of 10 µl.
Storage
4°C
Synonyms
N-Glycosidase A; PNGase A; Glycopeptidase A; N-linked-glycopeptide-(N-acetyl-β-D-glucosaminyl)-L-asparagine amidohydrolase; PNGase
Download
Download Datasheet"PNGase F" Total Products Page
| Catalog | Product Name | EC No. | CAS No. | Source | Price |
|---|---|---|---|---|---|
| NATE-0600 | Native Almonds Glycopeptidase A | EC 3.5.1.52 | 83534-39-8 | Almonds | Inquiry |
| NATE-0601 | Native Elizabethkingia meningoseptica PNGase F | EC 3.5.1.52 | 83534-39-8 | Elizabethkingia meningoseptica | Inquiry |
| NATE-0602 | Native Elizabethkingia miricola PNGase F | EC 3.5.1.52 | 83534-39-8 | Elizabethkingia miricola | Inquiry |
| NATE-0603 | Native Flavobacterium meningosepticum PNGase F | EC 3.5.1.52 | 83534-39-8 | Flavobacterium meningosepticum | Inquiry |