Description
In enzymology, a peptide-N4-(N-acetyl-beta-glucosaminyl) asparagine amidase (EC 3.5.1.52) is an enzyme that catalyzes a chemical reaction that cleaves a N4-(acetyl-beta-D-glucosaminyl)asparagine residue in which the glucosamine residue may be further glycosylated, to yield a (substituted) N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue. This enzyme belongs to the family of hydrolases, specifically those acting on carbon-nitrogen bonds other than peptide bonds in linear amides.
Abbr
PNGase F, Native (Flavobacterium meningosepticum)
Source
Flavobacterium meningosepticum
Applications
Glycoprotein analysis Removal of high mannose, hybrid, and complex N-glycans from glycoproteins Free of contaminants (Endo F, proteases, etc.)
Product Overview
PNGase F is purified from Flavobacterium meningosepticum (3) and it is free of proteases and Endo F activities. The following reagents are supplied with this product: Glycoprotein Denaturing Buffer, 10X GlycoBuffer 2, 10X NP-40, 10%
Enzyme Commission Number
EC 3.5.1.52
Activity
500,000 units/ml
Unit Definition
One unit is defined as the amount of enzyme required to remove > 95% of the carbohydrate from 10 µg of denatured RNase B in 1 hour at 37°C in a total reaction volume of 10 µl (65 NEB units = 1 IUB milliunit).
Stability
Storage Conditions: 20 mM Tris-HCl, 50 mM NaCl, 5 mM Na2EDTA, 50% Glycerol, pH 7.5 25°C Heat Inactivation: 75°C for 10 min
Synonyms
glycopeptide N-glycosidase; glycopeptidase; N-oligosaccharide glycopeptidase; N-glycanase; glycopeptidase; Jack-bean glycopeptidase; PNGase A; PNGase F; glycopeptide N-glycosidase; peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase; EC 3.5.1.52; PNGase F; 83534-39-8
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