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Nitrile hydratase from Sinorhizobium meliloti, Recombinant
Cat No.
NATE-1219
Description
In enzymology, nitrile hydratases (NHases; EC 4.2.1.84) are mononuclear iron or non-corrinoid cobalt enzymes that catalyse the hydration of diverse nitriles to their corresponding amides: R-C≡N + H2O → R-C(O)NH2.
Abbr
Nhase, Recombinant (Sinorhizobium meliloti)
Alias
Nhase
Source
Sinorhizobium meliloti 1021
Form
Supplied in 3.2 M ammonium sulphate
Enzyme Commission Number
EC 4.2.1.84
Activity
33.82 U/mg
CAS No.
82391-37-5
Molecular Weight
16126.0 Da (α-subunit), 24098.0 Da (β-subunit)
Purity
> 95 % as judged by SDS-PAGE
Concentration
165.7 U/ml
Unit Definition
One unit is defined as the amount of enzyme required to release 1 μmol of methacrylamide from methocrylonitrile (10 mM) per minute in 100 mM KH2PO4 buffer, pH 7.0, at 25°C, as measured at 224 nm.
Storage
Store at 4°C (shipped at room temperature)
Preparation Instructions
Agitate vial sufficiently to fully homogenise enzyme precipitate before use.
Synonyms
Nitrilase; 3-cyanopyridine hydratase; NHase; L-NHase; H-NHase; acrylonitrile hydratase; aliphatic nitrile hydratase; nitrile hydro-lyase; aliphatic-amide hydro-lyase (nitrile-forming)
Download
Download Datasheet| Catalog | Product Name | EC No. | CAS No. | Source | Price |
|---|---|---|---|---|---|
| NATE-1852 | Nitrile Hydratase (Crude Enzyme) | EC 4.2.1.84 | 82391-37-5 | E. coli | Inquiry |
| NATE-1217 | Nitrile hydratase from Rhodococcus erythropolis, Recombinant | EC 4.2.1.84 | 82391-37-5 | Rhodococcus erythropolis AJ270 | Inquiry |
| NATE-1218 | Nitrile hydratase from Rhodopseudomonas palustris, Recombinant | EC 4.2.1.84 | 82391-37-5 | Rhodopseudomonas palustris CGA009 | Inquiry |
| NATE-1626 | Nitrile hydratase from Recombinant microoganism | EC 4.2.1.84 | 82391-37-5 | Inquiry |