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Nitrile hydratase from Rhodococcus erythropolis, Recombinant
Cat No.
NATE-1217
Description
In enzymology, nitrile hydratases (NHases; EC 4.2.1.84) are mononuclear iron or non-corrinoid cobalt enzymes that catalyse the hydration of diverse nitriles to their corresponding amides: R-C≡N + H2O → R-C(O)NH2.
Abbr
Nhase, Recombinant (Rhodococcus erythropolis)
Alias
Nhase
Source
Rhodococcus erythropolis AJ270
Form
Supplied as a freeze-dried CFE powder.
Enzyme Commission Number
EC 4.2.1.84
Activity
> 4 U/mg (freeze-dried CFE)
CAS No.
82391-37-5
Molecular Weight
23487.3 Da (α-subunit), 25159.4 Da (β-subunit)
Purity
> 95 % as judged by SDS-PAGE
Unit Definition
One unit is defined as the amount of enzyme required to release 1 μmol of methacrylamide from methocrylonitrile (10 mM) per minute in 100 mM KH2PO4 buffer, pH 7.0, at 25°C, as measured at 224 nm.
Storage
Store at -20°C (shipped at ambient temperature)
Preparation Instructions
Agitate vial sufficiently to fully homogenise enzyme precipitate before use.
Synonyms
Nitrilase; 3-cyanopyridine hydratase; NHase; L-NHase; H-NHase; acrylonitrile hydratase; aliphatic nitrile hydratase; nitrile hydro-lyase; aliphatic-amide hydro-lyase (nitrile-forming)
Download
Download Datasheet| Catalog | Product Name | EC No. | CAS No. | Source | Price |
|---|---|---|---|---|---|
| NATE-1852 | Nitrile Hydratase (Crude Enzyme) | EC 4.2.1.84 | 82391-37-5 | E. coli | Inquiry |
| NATE-1218 | Nitrile hydratase from Rhodopseudomonas palustris, Recombinant | EC 4.2.1.84 | 82391-37-5 | Rhodopseudomonas palustris CGA009 | Inquiry |
| NATE-1219 | Nitrile hydratase from Sinorhizobium meliloti, Recombinant | EC 4.2.1.84 | 82391-37-5 | Sinorhizobium meliloti 1021 | Inquiry |
| NATE-1626 | Nitrile hydratase from Recombinant microoganism | EC 4.2.1.84 | 82391-37-5 | Inquiry |