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Nitrile hydratase from Rhodococcus erythropolis, Recombinant

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Cat No.

NATE-1217

Description

In enzymology, nitrile hydratases (NHases; EC 4.2.1.84) are mononuclear iron or non-corrinoid cobalt enzymes that catalyse the hydration of diverse nitriles to their corresponding amides: R-C≡N + H2O → R-C(O)NH2.

Abbr

Nhase, Recombinant (Rhodococcus erythropolis)

Alias

Nhase

Source

Rhodococcus erythropolis AJ270

Form

Supplied as a freeze-dried CFE powder.

Enzyme Commission Number

EC 4.2.1.84

Activity

> 4 U/mg (freeze-dried CFE)

CAS No.

82391-37-5

Molecular Weight

23487.3 Da (α-subunit), 25159.4 Da (β-subunit)

Purity

> 95 % as judged by SDS-PAGE

Unit Definition

One unit is defined as the amount of enzyme required to release 1 μmol of methacrylamide from methocrylonitrile (10 mM) per minute in 100 mM KH2PO4 buffer, pH 7.0, at 25°C, as measured at 224 nm.

Storage

Store at -20°C (shipped at ambient temperature)

Preparation Instructions

Agitate vial sufficiently to fully homogenise enzyme precipitate before use.

Synonyms

Nitrilase; 3-cyanopyridine hydratase; NHase; L-NHase; H-NHase; acrylonitrile hydratase; aliphatic nitrile hydratase; nitrile hydro-lyase; aliphatic-amide hydro-lyase (nitrile-forming)

"Nhase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
NATE-1852	Nitrile Hydratase (Crude Enzyme)	EC 4.2.1.84	82391-37-5	E. coli	Inquiry
NATE-1218	Nitrile hydratase from Rhodopseudomonas palustris, Recombinant	EC 4.2.1.84	82391-37-5	Rhodopseudomonas palustris CGA009	Inquiry
NATE-1219	Nitrile hydratase from Sinorhizobium meliloti, Recombinant	EC 4.2.1.84	82391-37-5	Sinorhizobium meliloti 1021	Inquiry
NATE-1626	Nitrile hydratase from Recombinant microoganism	EC 4.2.1.84	82391-37-5		Inquiry

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