Description
β-glucosidase is a glucosidase enzyme located in on the brush border of the small intestine that acts upon β1->4 bonds linking two glucose or glucose-substituted molecules (i.e., the disaccharide cellobiose). It is one of the cellulases, enzymes involved in the decomposition of cellulose and related polysaccharides; more specifically, an exocellulase with specificity for a variety of beta-D-glycoside substRates. It catalyzes the hydrolysis of terminal non-reducing residues in beta-D-glucosides with release of glucose.
Abbr
β-Glucosidase, Native (Thermotoga neopolitana)
Source
Thermotoga neopolitana
Applications
The enzyme may be used for hydrolysis of various glycosides such as polyphenol glycosides including naturally occurring antioxidants such as quercetin-glycosides found in various vegetables. For complete hydrolysis of 1 μmol of quercetin-4-glycoside in 5 minutes at 80°C and pH 5.5, about 28 pmol (~25 μg) enzyme was needed (Turner et al 2006)
Enzyme Commission Number
EC 3.2.1.6
Structure
The crystal structure of β-glucosidase from Thermotoga neopolitana has been determined to 2.05 Å resolution (Pozzo et al. 2010). – PDB entry 2X41
Unit Definition
One unit (U) of enzyme activity is the amount that leads to the release of 1 μmol of p-nitrophenyl from p-nitrophenyl-β-D-glucanopyranoside (pNPG) per minute.
Optimum temperature
the enzyme has optimum activity around 90°C
Synonyms
β-glucosidase; glycoside hydrolase; β-D-glucoside glucohydrolase; EC 3.2.1.6; gentiobiase; cellobiase; emulsin; elaterase; aryl-β-glucosidase; β-D-glucosidase; arbutinase; amygdalinase; p-nitrophenyl β-glucosidase; primeverosidase; amygdalase; linamarase; salicilinase; β-1,6-glucosidase
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