Description
Staphopains A and B are part of the staphylococcal proteolytic cascade (SPC) and may contribute to tissue invasion and metastatic infections of Staphylococcus aureus. Staphopain A, along with Staphopain B, may contribute to septic shock through the release of bradykinin and S-kinin from human kininogens. This activity required proteolytically-active Staphopain A and was augmented by Staphopain B.
Abbr
ScpA, Native (Staphylococcus aureus)
Source
Staphylococcus aureus
Applications
Staphopain A was used for cleaving the N terminus of atypical chemokine receptor (ACKR2) to suppress its ligand internalization activity in a study on the pivotal role of ACKR2 in ligand binding, internalization and scavenging of inflammatory responses.
Product Overview
Staphopain A is inhibited by Staphostatin A, E-64, and heavy metal ions such as HG2+ and AG+. Staphopain A is also inhibited by plasma α2-macroglobulin but not by human Cystatin C. Staphopain A and Staphopain B, members of the peptidase C47 family, are the major cysteine proteases secreted by S. aureus.
Enzyme Commission Number
EC 3.4.22.48
Molecular Weight
mol wt ~20 kDa by SDS-PAGE
Warnings
Lyophilized from a solution containing 25 mM sodium acetate pH 5.5, 100 mM NaCl, and 0.5 mM EDTA. Each vial contains 0.1 mg of protein. Reconstitute with 0.2 mL of water to create a 0.5 mg/mL solution.
Synonyms
Staphopain; EC 3.4.22.48; staphylopain; 347841-89-8; ScpA; Staphopain A; Staphylococcal cysteine proteinase A; Staphylopain A
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