Description
COX-1 catalyzes the conversion of arachidonic acid to prostaglandin H2 (the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins). It is involved in the homeostatic role of eicosanoids and constitutively almost all animal tissues. Has an apparent KM of 8.3 μM for arachidonic acid.
Abbr
COX-1, Native (Sheep)
Form
aqueous solution. Solution in 80 mM Tris-HCl, pH 8, with 0.1% TWEEN 20 and 300 μM diethyldithiocarbamate.
Enzyme Commission Number
EC 1.14.99.1
Activity
> 40,000 units/mg protein
Molecular Weight
dimer subunit mol wt 70 kDa
Unit Definition
One unit consumes one nanomole of oxygen per minute at 37°C in 0.1 M Tris-HCl buffer, pH 8, containing 100 μM arachidonate, 5 mM EDTA, 2 mM phenol, and 1 μM hematin.
Pathway
Alzheimer's disease, organism-specific biosystem; Cytochrome c oxidase, organism-specific biosystem; Huntington's disease, conserved biosystem
Synonyms
COX-1; Constitutive cyclooxygenase; Prostaglandin H synthase 1; Prostaglandin endoperoxide synthase; EC 1.14.99.1; prostaglandin synthase; prostaglandin G/H synthase; (PG)H synthase; PG synthetase; prostaglandin synthetase; fatty acid cyclooxygenase; prostaglandin endoperoxide synthetase
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