Description
COX-1 catalyzes the conversion of arachidonic acid to prostaglandin H2 (the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins). It is involved in the homeostatic role of eicosanoids and constitutively almost all animal tissues. Has an apparent KM of 8.3 μM for arachidonic acid.
Abbr
COX-1, Recombinant (Human)
Form
80 mM Tris, pH 8.0, containing 0.1% polysorbate 20, 300 µM DDC, and 10% glycerol
Activity
>20,000 units/mg
Unit Definition
One unit is defined as the amount of enzyme required to consume 1 nmol of oxygen per minute at 37°C in 100 mM Tris, pH 8.0, containing 100 µM arachidonate, 5 mM EDTA, 2 mM phenol, and 1 µM hematin.
Storage
-80°C (as supplied); avoid freeze/thaw cycles by aliquoting protein
Synonyms
COX-1; Constitutive cyclooxygenase; Prostaglandin H synthase 1; Prostaglandin endoperoxide synthase; EC 1.14.99.1; prostaglandin synthase; prostaglandin G/H synthase; (PG)H synthase; PG synthetase; prostaglandin synthetase; fatty acid cyclooxygenase; prostaglandin endoperoxide synthetase
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