Description
Pectinases hydrolyses pectin, which is a component of the cell wall. They may attack methyl-esterified pectin or de-esterified pectin. It is a source of pectinase activity, also containing cellulase and hemicellulase activities.
Abbr
Pectinase, Native (Rhizopus sp.)
Applications
Used in plant protoplast preparation to digest cell wall prior to organelle isolation. Petctinase is an enzyme from Rhizopus sp. that is used in plant protoplast preparation to digest cell wall prior to organelle isolation. It has been used to digest the cell wall of Arabidopsis root cells to study peroxin 16 in peroxisomes and endoplasmic reticulum. Pectinases are used to study their role in the invasion of plant tissues by phytopathogens, the spoilage of produce and various food processing and plant biotechnology applications.
Enzyme Commission Number
EC 3.2.1.15
Activity
> 5 units/mg protein (Lowry)
Unit Definition
One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 4.0 at 25°C.
Synonyms
Pectinase; pectin depolymerase; endopolygalacturonase; pectolase; pectin hydrolase; pectin polygalacturonase; endo-polygalacturonase; poly-α-1,4-galacturonide glycanohydrolase; endogalacturonase; endo-D-galacturonase; poly (1,4-α-D-galacturonide) glycanohydrolase; polygalacturonase; EC 3.2.1.15; 9032-75-1
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