Description
Pectolytic enzyme preparation produced from a selected strain of Aspergillus niger:contains mainly pectintranseliminase, polygalacturonase, and pectinesterase and small amounts of hemicellulases and cellulases. Pectinases hydrolyses pectin, which is a component of the cell wall. They may attack methyl-esterified pectin or de-esterified pectin. It is a source of pectinase activity, also containing cellulase and hemicellulase activities.Pectinase catalyzes the random hydrolysis of a-(1-4)-Dgalactosiduronic linkages in pectin and other galacturonans.
Abbr
Pectinase, Native (Aspergillus niger)
Applications
Used in plant protoplast preparation to digest cell wall prior to organelle isolation. Petctinase is an enzyme from Aspergillus niger that is used in plant protoplast preparation to digest cell wall prior to organelle isolation. It has been used to conduct partial saccharification of sugars. Pectinases are used to study their role in the invasion of plant tissues by phytopathogens, the spoilage of produce and various food processing and plant biotechnology applications. This product is suitable for plant cell culture and is provided in an aqueous glycerol solution.
Form
Solution in 40% glycerol
Enzyme Commission Number
EC 3.2.1.15
Activity
> 5 units/mg protein (Lowry)
Unit Definition
One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 4.0 at 25°C.
Synonyms
Pectinase; pectin depolymerase; endopolygalacturonase; pectolase; pectin hydrolase; pectin polygalacturonase; endo-polygalacturonase; poly-α-1,4-galacturonide glycanohydrolase; endogalacturonase; endo-D-galacturonase; poly (1,4-α-D-galacturonide) glycanohydrolase; polygalacturonase; EC 3.2.1.15; 9032-75-1
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