Description
In enzymology, a 4-hydroxybenzoate 3-monooxygenase (EC 1.14.13.2) is an enzyme that catalyzes the chemical reaction:4-hydroxybenzoate + NADPH + H+ + O2↔ protocatechuate + NADP+ + H2O. The 4 substrates of this enzyme are 4-hydroxybenzoate, NADPH, H+, and O2, whereas its 3 products are protocatechuate, NADP+, and H2O. This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of one atom o oxygen into the other donor.
Abbr
p-Hydroxybenzoate Hydroxylase, Native (Pseudomonas sp.)
Applications
This enzyme is useful for enzymatic determination of choline esterase when coupled with prot ocatechuate 3, 4-dioxygenase.
Form
lyophilized powder; Contains mannitol and stabilizers
Enzyme Commission Number
EC 1.14.13.2
Activity
~20 units/mg solid
Molecular Weight
mol wt 55-~60 kDa
pH Stability
pH 5.0-7.5 (25°C, 72hr)
Michaelis Constant
2.0 x 10-5M (p-Hydroxybenzoate), 4.0 x 10-5M (NADPH)
Structure
One mol of FAD per mol of enzyme
Unit Definition
One unit will hydroxylate 1.0 μmole of p-hydroxybenzoate to protecatechuate per min at pH 8.2 at 37°C in the presence of NADPH.
Thermal stability
below 40°C (pH 6.0, 15min)
Inhibitors
Ag+, Hg++, PCMB, SDS
Synonyms
p-hydroxybenzoate hydrolyase; p-hydroxybenzoate hydroxylase; 4-hydroxybenzoate 3-hydroxylase; 4-hydroxybenzoate monooxygenase; 4-hydroxybenzoic hydroxylase; p-hydroxybenzoate-3-hydroxylase; p-hydroxybenzoic acid hydrolase; p-hydroxybenzoic acid hydroxylase; p-hydroxybenzoic hydroxylase; EC 1.14.13.2; 9059-23-8
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