Description
In enzymology, a 4-hydroxybenzoate 3-monooxygenase (EC 1.14.13.2) is an enzyme that catalyzes the chemical reaction: 4-hydroxybenzoate + NADPH + H+ + O2 ↔ protocatechuate + NADP+ + H2O. The 4 substrates of this enzyme are 4-hydroxybenzoate, NADPH, H+, and O2, whereas its 3 products are protocatechuate, NADP+, and H2O. This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of one atom o oxygen into the other donor. This enzyme participates in benzoate degradation via hydroxylation and 2,4-dichlorobenzoate degradation. It employs one cofactor, FAD.
Abbr
P-hydroxybenzoate hydroxylase (Microorganism)
Applications
This enzyme is useful for enzymatic determination of choline esterase when coupled with protocatechuate 3, 4-dioxygenase.
Appearance
Yellowish amorphous powder, lyophilized
Enzyme Commission Number
EC 1.14.13.2
Activity
GradeⅢ 20U/mg-solid or more (containing approx. 40% of stabilizers)
Contaminants
NADPH oxidase < 1.0×10⁻¹%
Molecular Weight
55 kDa~60 kDa
pH Stability
pH 5.0-7.5 (25°C, 72hr)
Michaelis Constant
2.0×10⁻⁵M (p-Hydroxybenzoate), 4.0×10⁻⁵M (NADPH)
Structure
One mol of FAD per mol of enzyme
Thermal stability
below 40°C (pH 6.0, 15min)
Stability
Stable at-20°C for at least one year
Inhibitors
Ag⁺, Hg⁺⁺, PCMB, SDS
Synonyms
4-hydroxybenzoate; NADPH: oxygen oxidoreductase (3-hydroxylating); p-hydroxybenzoate hydrolyase; p-hydroxybenzoate hydroxylase; 4-hydroxybenzoate 3-hydroxylase; 4-hydroxybenzoate monooxygenase; 4-hydroxybenzoic hydroxylase; p-hydroxybenzoate-3-hydroxylase; p-hydroxybenzoic acid hydrolase; p-hydroxybenzoic acid hydroxylase; p-hydroxybenzoic hydroxylase; EC 1.14.13.2
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