Description
In enzymology, an aminoacylase (EC 3.5.1.14) is an enzyme that catalyzes the chemical reaction:N-acyl-L-amino acid + H2O↔ carboxylate + L-amino acid. Thus, the two substRates of this enzyme are N-acyl-L-amino acid and H2O, whereas its two products are carboxylate and L-amino acid. This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. This enzyme participates in urea cycle and metabolism of amino groups.
Abbr
ACY1, Native (Porcine)
Applications
Acylase I from porcine kidney has been used to study the acylase I-catalyzed deacetylation of various S-alkyl-N-acetyl-L-cysteines and their carbon and oxygen analogues. Acylase I may be useful to catalyze N-acetyl amino acids to enantiomerically pure L-amino acids.
Product Overview
Protein determined by biuret.
Enzyme Commission Number
EC 3.5.1.14
Activity
> 2,000 units/mg protein; 500-1,500 units/mg protein
Unit Definition
One unit will hydrolyze 1.0 μmole of N-acetyl-L-methionine per hr at pH 7.0 at 25°C.
Pathway
2-Oxocarboxylic acid metabolism, organism-specific biosystem; Aflatoxin activation and detoxification, organism-specific biosystem; Biological oxidations, organism-specific biosystem
Function
aminoacylase activity; metal ion binding; metallopeptidase activity
Synonyms
aminoacylase 1; aminoacylase I; dehydropeptidase II; histozyme; hippuricase; benzamidase; acylase I; hippurase; amido acid deacylase; L-aminoacylase; acylase; aminoacylase; L-amino-acid acylase; α-N-acylaminoacid hydrolase; long acyl amidoacylase; short acyl amidoacylase; ACY1 (gene name); N-acyl-L-amino-acid amidohydrolase; EC 3.5.1.14; 9012-37-7
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