Description
Bromelain is a cysteine endopeptidase with broad specificity for cleavage of proteins. Bromelain may be from a stem or piece of fruit. Stem bromelain (SBM) (EC 3.4.22.32), a proteolytic enzyme, is a widely accepted phytotherapeutical drug member of the bromelain family of proteolytic enzymes obtained from Ananas comosus. Some of the therapeutic benefits of SBM are reversible inhibition of platelet aggregation, angina pectoris, bronchitis, sinusitis, surgical traumas, thrombophlebitis, pyelonephritis and enhanced absorption of drugs, particularly of antibiotics. Its anti-metastasis and anti-inflammatory activities are apparently independent of its proteolytic activity.
Abbr
SBM, Native (Pineapple)
Alias
ananase; bromelase; bromelin; extranase
Applications
Bromelain may be used to inhibit the biosysnthesis of proinflammatory prostaglandins. It may be used to reduce clotting efficiency. Bromelain, from pineapple stem, has been used to make enzymatic hydrolysates of honeybee-collected pollen.
Form
Lyophilized powder containing mannitol and potassium phosphate buffer salts
Enzyme Commission Number
EC 3.4.22.32
Activity
> 3 units/mg protein; 5-15 units/mg protein
Composition
Protein, > 35% biuret
Unit Definition
One unit will release 1.0 micromole of p-nitrophenol from N-alpha-CBZ-L-Lysine p-nitrophenyl ester per minute at pH 4.6 at 25°C. One old titrimetric unit (pH 4.5 at 45°C) is equivalent to approximately 1.7 new units (pH 4.6 at 25°C).
Buffer
The product may be suspended in acetate buffer, pH 4.5 at 1 mg/mL concentration, yielding a hazy, off-white suspension.
Synonyms
stem bromelain; EC 3.4.22.32; 37189-34-7; bromelain; pineapple stem bromelain; SBM
Download Datasheet