Description
Pyruvate Oxidase consists of four subunits with identical molecular weights. PoxB reacts with certain aldehydes and phosphate can be replaced by arsenate. Oxygen as well as several artificial compounds can function as electron acceptors. Pyruvate Oxidase is activated by phospholipids as well as monomeric and micellar amphiphiles.
Abbr
PoxB, Native (Microorganisms)
Applications
Pyruvate Oxidase (PoxB) converts pyruvate directly to acetate and CO2. It is used to study pyruvate metabolism. It is used to study aerobic metabolism of bacterium, such as Lactobacillus plantarumand Strept oc occus pneumoniae. Pyruvate Oxidase is used for enzymatic determination of pyruvate, GOT, and GPT in clinical analysis.
Form
Lyophilized powder containing FAD and sugar as stabilizer
Enzyme Commission Number
EC 1.2.3.3
Activity
> 1.5 units/mg; > 35 units/mg protein (biuret)
Molecular Weight
mol wt ~260 kDa
pH Stability
pH 5.7-6.5 (25°C, 20hr)
Michaelis Constant
3.4 X 10-4M (Pyruvate)
Unit Definition
One unit will produce 1.0 μmole of H2O2 per min during the conversion of pyruvate and phosphate to acetylphosphate and CO2 at pH 5.7 at 37°C.
Thermal stability
below 45°C (pH 6.0, 15min)
Inhibitors
Fe++,Zn++,Cu++,Ag+,Hg++
Synonyms
pyruvate oxidase; EC 1.2.3.3; pyruvic oxidase; phosphate-dependent pyruvate oxidase; 9001-96-1; Pyruvate:oxygen oxidoreductase (phosphorylating); PoxB
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