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Native Microorganism Pyruvate oxidase
Cat No.
DIA-215
Description
In enzymology, a pyruvate oxidase (EC 1.2.3.3) is an enzyme that catalyzes the chemical reaction: pyruvate + phosphate + O2 ↔ acetyl phosphate + CO2 + H2O2. The 3 substrates of this enzyme are pyruvate, phosphate, and O2, whereas its 3 products are acetyl phosphate, CO2, and H2O2. This enzyme belongs to the family of oxidoreductases, specifically those acting on the aldehyde or oxo group of donor with oxygen as acceptor. This enzyme participates in pyruvate metabolism. It has 2 cofactors: FAD, and Thiamin diphosphate.
Abbr
Pyruvate oxidase (Microorganism)
Alias
pyruvic oxidase
Source
Microorganism
Applications
This enzyme is useful for enzymatic determination of pyruvate, GOT, GPT in clinical analysis.
Appearance
Yellowish amorphous powder, lyophilized
Enzyme Commission Number
EC 1.2.3.3
Activity
GradeⅢ 1.5U/mg-solid or more
CAS No.
9001-96-1
Contaminants
ATPase < 5.0×10⁻²% GOT, GPT < 5.0×10⁻²%
Molecular Weight
approx. 260 kDa
Isoelectric point
4.3
pH Stability
pH 5.7-6.5 (25°C, 20hr)
Michaelis Constant
3.4×10⁻⁴M (Pyruvate)
Optimum pH
5.7
Optimum temperature
65°C
Thermal stability
below 45°C (pH 6.0, 15min)
Stability
Stable at-20°C for at least one year
Stabilizers
Sugars, FAD
Inhibitors
Fe⁺⁺,Zn⁺⁺,Cu⁺⁺,Ag⁺,Hg⁺⁺
Synonyms
EC 1.2.3.3; pyruvate: oxygen 2-oxidoreductase (phosphorylating); pyruvic oxidase; phosphate-dependent pyruvate oxidase
Download
Download Datasheet"Pyruvate oxidase" Total Products Page
| Catalog | Product Name | EC No. | CAS No. | Source | Price |
|---|---|---|---|---|---|
| DIA-169 | Native Aerococcuss viridans Pyruvate oxidase | EC 1.2.3.3 | 9001-96-1 | Aerococcuss viridans | Inquiry |
| DIA-289 | Pyruvate Oxidase from E.coli, Recombinant | 9001-96-1 | E. coli | Inquiry | |
| NATE-0613 | Native Microorganisms Pyruvate Oxidase | EC 1.2.3.3 | 9001-96-1 | Microorganisms | Inquiry |
| EXWM-1210 | pyruvate oxidase | EC 1.2.3.3 | 9001-96-1 | Inquiry |