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Native Microorganism Pyruvate oxidase

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Cat No.

DIA-215

Description

In enzymology, a pyruvate oxidase (EC 1.2.3.3) is an enzyme that catalyzes the chemical reaction: pyruvate + phosphate + O2 ↔ acetyl phosphate + CO2 + H2O2. The 3 substrates of this enzyme are pyruvate, phosphate, and O2, whereas its 3 products are acetyl phosphate, CO2, and H2O2. This enzyme belongs to the family of oxidoreductases, specifically those acting on the aldehyde or oxo group of donor with oxygen as acceptor. This enzyme participates in pyruvate metabolism. It has 2 cofactors: FAD, and Thiamin diphosphate.

Abbr

Pyruvate oxidase (Microorganism)

Alias

pyruvic oxidase

Source

Microorganism

Applications

This enzyme is useful for enzymatic determination of pyruvate, GOT, GPT in clinical analysis.

Appearance

Yellowish amorphous powder, lyophilized

Enzyme Commission Number

EC 1.2.3.3

Activity

GradeⅢ 1.5U/mg-solid or more

CAS No.

9001-96-1

Contaminants

ATPase < 5.0×10⁻²% GOT, GPT < 5.0×10⁻²%

Molecular Weight

approx. 260 kDa

Isoelectric point

4.3

pH Stability

pH 5.7-6.5 (25°C, 20hr)

Michaelis Constant

3.4×10⁻⁴M (Pyruvate)

Optimum pH

5.7

Optimum temperature

65°C

Thermal stability

below 45°C (pH 6.0, 15min)

Stability

Stable at-20°C for at least one year

Stabilizers

Sugars, FAD

Inhibitors

Fe⁺⁺,Zn⁺⁺,Cu⁺⁺,Ag⁺,Hg⁺⁺

Synonyms

EC 1.2.3.3; pyruvate: oxygen 2-oxidoreductase (phosphorylating); pyruvic oxidase; phosphate-dependent pyruvate oxidase

"Pyruvate oxidase" Total Products Page

Enzymes

Catalog	Product Name	EC No.	CAS No.	Source	Price
DIA-169	Native Aerococcuss viridans Pyruvate oxidase	EC 1.2.3.3	9001-96-1	Aerococcuss viridans	Inquiry
DIA-289	Pyruvate Oxidase from E.coli, Recombinant		9001-96-1	E. coli	Inquiry
NATE-0613	Native Microorganisms Pyruvate Oxidase	EC 1.2.3.3	9001-96-1	Microorganisms	Inquiry
EXWM-1210	pyruvate oxidase	EC 1.2.3.3	9001-96-1		Inquiry

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