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Native Jack bean Urease
Cat No.
PHAM-180
Description
Ureases (EC 3.5.1.5), functionally, belong to the superfamily of amidohydrolases and phosphotriesterases. It is an enzyme that catalyzes the hydrolysis of urea into carbon dioxide and ammonia. The reaction occurs as follows: (NH2)2CO + H2O → CO2 + 2NH3.
Abbr
Urease (Jack bean)
Alias
Urease
Source
Jack bean
Applications
This enzyme is useful for enzymatic determination of urea in clinical analysis.
Appearance
White amorphous powder, lyophilized
Form
Freeze dried powder
Enzyme Commission Number
EC 3.5.1.5
Activity
GradeⅡ(-201) 100U/mg-solid or more
CAS No.
9002-13-5
Contaminants
Asparaginase < 2.0×10⁻²% Arginase < 2.0×10⁻³% NH₄⁺ < 5.0×10⁻⁴µg/U
Molecular Weight
approx. 480 kDa
Isoelectric point
5.0-5.1
pH Stability
pH 5.5-8.5 (30°C, 17hr)
Michaelis Constant
1.05×10⁻²M (Urea)
Structure
8 active sites with SH-groups per mole of the enzyme
Optimum pH
6
Optimum temperature
60°C
Thermal stability
below 50°C (pH 8.0, 60min)
Stability
Store at -20°C (A decrease in activity of ca.15% may occur within 6 months)
Stabilizers
EDTA, glutathione, succinate, BSA
Inhibitors
Heavy metal ions (Ag⁺,Hg⁺⁺,etc.)
Synonyms
EC 3.5.1.5; Urease
Download
Download Datasheet| Catalog | Product Name | EC No. | CAS No. | Source | Price |
|---|---|---|---|---|---|
| NATE-0923 | Urease, Recombinant | 9002-13-5 | E. coli | Inquiry | |
| NATE-1035 | Urease from Bacteria, Recombinant | EC 3.5.1.5 | 9002-13-5 | E. coli | Inquiry |
| PHAM-179 | Native H. pylori Urease | EC 3.5.1.5 | 9002-13-5 | H. pylori | Inquiry |
| EXWM-4438 | urease | EC 3.5.1.5 | 9002-13-5 | Inquiry |