Description
Ureases (EC 3.5.1.5), functionally, belong to the superfamily of amidohydrolases and phosphotriesterases. It is an enzyme that catalyzes the hydrolysis of urea into carbon dioxide and ammonia. The reaction occurs as follows: (NH2)2CO + H2O → CO2 + 2NH3.
Applications
This enzyme is useful for enzymatic determination of urea in clinical analysis.
Appearance
White amorphous powder, lyophilized
Enzyme Commission Number
EC 3.5.1.5
Activity
GradeⅡ(-201) 100U/mg-solid or more
Contaminants
Asparaginase < 2.0×10⁻²% Arginase < 2.0×10⁻³% NH₄⁺ < 5.0×10⁻⁴µg/U
Molecular Weight
approx. 480 kDa
Isoelectric point
5.0-5.1
pH Stability
pH 5.5-8.5 (30°C, 17hr)
Michaelis Constant
1.05×10⁻²M (Urea)
Structure
8 active sites with SH-groups per mole of the enzyme
Thermal stability
below 50°C (pH 8.0, 60min)
Stability
Store at -20°C (A decrease in activity of ca.15% may occur within 6 months)
Stabilizers
EDTA, glutathione, succinate, BSA
Inhibitors
Heavy metal ions (Ag⁺,Hg⁺⁺,etc.)
Synonyms
EC 3.5.1.5; Urease