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Native Flavobacterium meningosepticum Glycerol kinase
Cat No.
NATE-1155
Description
The activity of glycerol kinase is found widely in nature. In microorganisms GK makes possible the utilization of glycerol as a carbon source. In mammals the enzyme represents a juncture of sugar and fat metabolism; The enzyme is important to the clinical chemist in the determination of glycerol. GK is also useful in the assay of glyceraldehydes and dihydroxyacetone following their quantitative reduction to glycerol with sodium borohydride.
Abbr
GK, Native (Flavobacterium meningosepticum)
Alias
GK
Source
Flavobacterium meningosepticum
Applications
Useful for the measurement of Triglyceride.
Appearance
White to light grayish white amorphous powder, lyophilized.
Form
Freeze dried powder
Enzyme Commission Number
EC 2.7.1.30
Activity
More than 70 U/mg solid
Contaminants
Hexokinase < 0.05%; Catalase < 0.1%; ATPase < 0.01%; Myokinase < 0.05%
Molecular Weight
150 kDa (TSK G3000SWXL) 50 kDa (SDS‒PAGE)
Isoelectric point
4.3
pH Stability
5.0‒11.0
Michaelis Constant
Glycerol 8.8 × 10-5M ATP 3.0 × 10-5M
Unit Definition
One unit is defined as the amount of enzyme which converts 1 μmole of glycerol to glycerol‒3‒phosphate per minute at 37°C under the conditions specified in the assay procedure.
Optimum pH
8
Optimum temperature
80°C
Thermal stability
Stable at 60°C and below
Storage
Storage at -20°C in the presence of a desiccant is recommended.
Synonyms
glycerokinase; GK; ATP: glycerol-3-phosphotransferase; glycerol kinase phosphorylating; glyceric kinase; EC 2.7.1.30
Download
Download Datasheet| Catalog | Product Name | EC No. | CAS No. | Source | Price |
|---|---|---|---|---|---|
| DIA-149 | Native Microorganism Glycerol Kinase | EC 2.7.1.30 | 9030-66-4 | Microorganism | Inquiry |
| NATE-0286 | Native Bacillus stearothermophilus Glycerokinase | EC 2.7.1.30 | 9030-66-4 | Bacillus stearothermophilus | Inquiry |
| NATE-0287 | Native Cellulomonas sp. Glycerokinase | EC 2.7.1.30 | 9030-66-4 | Cellulomonas sp. | Inquiry |
| NATE-0288 | Native Escherichia coli Glycerokinase | EC 2.7.1.30 | 9030-66-4 | Escherichia coli | Inquiry |