Description
Glycerol kinase is a phosphotransferase enzyme involved in triglycerides and glycerophospholipids synthesis. Glycerol kinase catalyzes tge MgATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate and is the rate limiting enzyme in the utilization of glycerol. It is also subject to feedback regulation by fructose-1,6-bisphosphate.
Abbr
GK, Native (Cellulomonas sp.)
Applications
This enzyme is useful for enzymatic determination of glycerol and triglyceride when coupled with glycerol-3-phosphate dehydrogenase, glycerol-3-phosphate oxidase or pyruvate kinase and lactate dehydrogenase, lipoprotein lipase in clinical analysis.
Product Overview
The enzyme has the highest specificity for glycerol, and also phosphorylates dihydroxyacetone and glyceraldehyde. Mg++ is essentially required for the reaction.
Form
Lyophilized powder containing phosphate buffer salts and sodium gluconate
Enzyme Commission Number
EC 2.7.1.30
Activity
20 U/mg-solid or more
Molecular Weight
mol wt ~128 kDa ((by gel filtration)
pH Stability
pH 5.5 x 10.0 (25°C, 20hr)
Michaelis Constant
4.4 x 10-5M (Glycerol), 4.3 x 10-4M (ATP)
Unit Definition
One unit will convert 1.0 μmole of glycerol and ATP to L-α-glycerophosphate and ADP per min at pH 9.8 at 25°C in a coupled system with PK/LDH.
Optimum pH
9.8 (G-3-PDH system), 7.8 (G-3-P oxidase system)
Thermal stability
below 40°C (pH 7.5, 15min)
Inhibitors
p-Chloromercuribenzoate, heavy metal ions (Pb++, Fe++, Hg++, Ag+)
Synonyms
EC 2.7.1.30; glycerokinase; GK; ATP:glycerol-3-phosphotransferase; glycerol kinase (phosphorylating); glyceric kinase; 9030-66-4
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