Description
Chondroitinase B cleaves, via an elimination mechanism, polysaccharide chains containing 1-4 linkages between hexosamines and iduronic acid residues in dermatan sulfate (chondroitin B).The reaction yields oligosaccharide products (mainly disaccharides) containing unsaturated uronic acids which can be detected by UV spectroscopy at 232 nm.
Source
Flavobacterium heparinum
Applications
Research reagent (glycobiology, preparation of oligosaccharide libraries from dermatan sulfate).
Determination of contents of chondrotin sulfates by HPLC.
Enzyme Commission Number
EC 4.2.2.19
Activity
>200 IU/mg (substrate: dermatan sulfate)
Isoelectric point
9.4-9.6
Specificity
Dermatan sulfate (chondroitin sulfate B).
Unit Definition
One international unit (IU) of chondroitinase B is defined as the amount of enzyme that will liberate 1.0 μmole unsaturated oligosaccharides from dermatan sulfate per minute at 30 °C and pH 8.0.
Optimum temperature
20°C – 37°C
Stability
12 months frozen at -20°C in aqueous buffers containing sodium phosphate and sucrose 5%.
Synonyms
Chondroitinase B; EC 4.2.2.19; chondroitin B lyase; ChonB; ChnB
Download Datasheet