Description
In enzymology, a chondroitin B lyase (EC 4.2.2.19) is an enzyme that catalyzes the chemical reaction:Eliminative cleavage of dermatan sulfate containing 1,4-beta-D-hexosaminyl and 1,3-beta-D-glucurosonyl or 1,3-alpha-L-iduronosyl linkages to disaccharides containing 4-deoxy-beta-D-gluc-4-enuronosyl groups to yield a 4,5-unsaturated dermatan-sulfate disaccharide (deltaUA-GalNAc-4S). This enzyme belongs to the family of lyases, specifically those carbon-oxygen lyases acting on polysaccharides.
Abbr
ChonB, Native (Flavobacterium heparinum)
Source
Flavobacterium heparinum
Species
Flavobacterium heparinum
Applications
As research reagent (glycosaminoglycan degradation). For the preparation of di-and oligo-saccharides of dermatan sulfate.
Product Overview
The enzyme cleaves, via an elimination mechanism, polysaccharide chains containing 1-4 linkages between hexosamines and iduronic acid residues in dermatan sulfate (chondroitin sulfate B).The reaction yields oligosaccharide products (mainly disaccharides) containing unsaturated uronic acids which can be detected by UV spectroscopy at 232 nm.
Enzyme Commission Number
EC 4.2.2.19
Activity
> 550 IU/mg (substrate: dermatan sulfate)
Molecular Weight
54,779 Da
Purity
> 90 % by reversed phase HPLC analysis.
Isoelectric point
9.4 – 9.6
Specificity
Dermatan sulfate (chondroitin sulfate B).
Unit Definition
One international unit (IU) is defined as the amount of enzyme that will liberate 1.0 µmole unsaturated oligosaccharides from dermatan sulfate per minute at 30°C and pH 8.0.
Optimum pH
pH optimum for activity: 7-8; pH range for activity: 5-10
Optimum temperature
20°C – 37°C
Stability
Expiration is 30 months from manufacturing date, frozen at –70°C in aqueous buffers containing Sodium Chloride, Sodium Phosphate and Sucrose 5%.
Synonyms
Chondroitinase B; EC 4.2.2.19; chondroitin B lyase; ChonB; ChnB
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