Description
Oxidoreductase that catalyzes the demethylation of sarcosine to glycine. Use Sarcosine Oxidase in your preferred creatinine reagent mix and rely on the proven diagnostic quality of this product.
Abbr
SAO, Native (E. coli)
Applications
Use Sarcosine Oxidase in diagnostic tests for the determination of creatinine. This can be done using one of two methods: (1) In combination with Creatinase and Creatininase. (2) In combination with Creatinine Deaminase, N-Carbamoylsarcosine Amidase and N-Methylhydantoinase (ATP-hydrolyzing).
Appearance
Yellow lyophilizate
Activity
22-40 U/mg lyophilizate; >45 U/mg protein
Contaminants
ATPase: <0.01 Catalase: <10.0 Contaminating oxidases (FOX): <0.005 Creatinase: <0.001 Creatininase: <0.01 Creatinine deaminase: <0.001 N-Carbamoylsarcosine amidohydrolase: <0.001 N-Methylhydantoinase: <0.001
Michaelis Constant
at +25°C: 3.7 x 10-3 mol/l at +37°C: 6.3 x 10-3 mol/l
Specificity
Sarcosine Oxidase reacts with sarcosine (100%), N-ethylglycine, 2 mmol/l (4%), L(-)-proline (0.28%), carbamoylsarcosine (0%), and glycine (0%).
Thermal stability
Up to +50°C
Stability
At -15 to -25°C within specification range for 12 months. Store dry. Protect from light.
Inhibitors
Completely inhibited by ZnCl2 (7 mmol/l), CdCl2 (7 mol/l), heavy metals and NaN3. Chloroacetic amine (0.2%) does not inhibit.
Synonyms
Sarcosine Oxidase; SAO
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