Description
Sarcosine oxidase (SAO) is an enzyme that catalyzes the oxidative demethylation of sarcosine to yield glycine, H2O2, 5, 10-CH2-tetrahydrofolate in a reaction requiring H4-tetrahydrofolate and oxygen. sarcosine + H2O + O2 = glycine + formaldehyde + H2O2.
Applications
This enzyme is useful for enzymatic determination of creatinine, creatine, and sarcosine when coupling with creatinine amidohydrolase and creatine amidinohydrolase.-341 is newer type of sarosine oxidase, with improved stability in antimicrobial reagent.
Appearance
Yellowish amorphous powder, lyophilized
Enzyme Commission Number
EC 1.5.3.1
Activity
GradeⅢ 8.0U/mg-solid or more
Contaminants
Catalase < 1.0%
Molecular Weight
approx. 65 kDa (by gel filtration)
Isoelectric point
4.9±0.1
pH Stability
pH 6.5-9.0 (25°C, 24hr)
Michaelis Constant
2.8×10⁻³M (Sarcosine)
Optimum temperature
40-50°C
Thermal stability
below 55°C (pH 7.5, 10min)
Stability
Stable at-20°C for at least one year
Stabilizers
Potassium gluconate
Inhibitors
Cu⁺⁺, Ag⁺, Hg⁺⁺, p-chloromercuribenzoate, N-ethylmaleimide, SDS
Function
Amine oxidase activity; oxidoreductase activity; calcium ion binding; copper ion binding; quinone binding.
Synonyms
Sarcosine Oxidase; EC 1.5.3.1; SAO
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